色谱法
高效液相色谱法
分析物
分辨率(逻辑)
化学
亲水作用色谱法
反相色谱法
色谱柱
计算机科学
人工智能
作者
Mr. Uday R. Patond,Sachin C. Kale,Ashish Gawai,K. R. Biyani
出处
期刊:International Journal of Advanced Research in Science, Communication and Technology
[Naksh Solutions]
日期:2022-06-30
卷期号:: 545-557
被引量:5
标识
DOI:10.48175/ijarsct-5602
摘要
Chromatography, although primarily a separation technique, is mostly employed in chemical analysis in which High-performance liquid chromatography (HPLC) is an extremely versatile technique where analytes are separated by passage through a column packed with micro meter-sized particles. Now a day reversed-phase chromatography is the most commonly used separation technique in HPLC. The reasons for this include the simplicity, versatility, and scope of the reversed-phase method as it is able to handle compounds of a diverse polarity and molecular mass. Reversed phase chromatography has found both analytical and preparative applications in the area of biochemical separation and purification. Molecules that possess some degree of hydrophobic character, such as proteins, peptides and nucleic acids, can be separated by reversed phase chromatography with excellent recovery and resolution. This review covers the importance of RP-HPLC in analytical method development and their strategies along with brief knowledge of critical chromatographic parameters need to be optimized for an efficient method development
科研通智能强力驱动
Strongly Powered by AbleSci AI