结合
高碘酸盐
共轭体系
化学
共价键
产量(工程)
多糖
组合化学
色谱法
生物化学
有机化学
材料科学
聚合物
数学分析
数学
冶金
出处
期刊:Vaccine
[Elsevier BV]
日期:2009-06-25
卷期号:27 (46): 6468-6470
被引量:123
标识
DOI:10.1016/j.vaccine.2009.06.013
摘要
A conjugate can be a polysaccharide (PS) covalently attached to a protein, which provides T cell epitopes for a normally T cell independent antigen. To produce a conjugate vaccine, the purified PS must first be chemically modified to generate reactive groups that can link to the protein. Two commonly used methods for PS activation are periodate oxidation at vicinal hydroxyls and cyanylation of hydroxyls. The PS should be of known molecular size before and after activation. Low molecular weight impurities in the protein may result in inefficient conjugation. Two critical measures after conjugation and purification are the PS to protein ratio and the percent non-conjugated saccharide (free saccharide). Yield and conjugate stability are critical considerations. Typically, considerably less than 20% of the activated PS becomes conjugated. Yield can be improved using newer conjugation methods, whereby highly reactive groups are generated on both the PS and carrier protein with yields approaching 50%. Two major measures used to follow vaccine stability are changes in molecular size and percent free (unbound) PS.
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