Structure of a membrane-attack complex/perforin (MACPF) family protein from the human gut symbiontBacteroides thetaiotaomicron

生物 穿孔素 补体膜攻击复合物 拟杆菌 细胞生物学 跨膜蛋白 背景(考古学) 遗传学 拟杆菌 细菌 免疫系统 补体系统 古生物学 CD8型 受体
作者
Qingping Xu,Polat Abdubek,Tamara Astakhova,Herbert L. Axelrod,Constantina Bakolitsa,Xiaohui Cai,Dennis Carlton,Connie Chen,Hsiu-Ju Chiu,Thomas Clayton,Debanu Das,Marc C. Deller,Lian Duan,Kyle Ellrott,Carol L. Farr,Julie Feuerhelm,Joanna C. Grant,Anna Grzechnik,Gye Won Han,Lukasz Jaroszewski,Kevin K. Jin,Heath E. Klock,Mark W. Knuth,Piotr Koźbiał,Srikrishna Subramanian,Abhinav Kumar,W.W.Y. Lam,David Marciano,Mitchell D. Miller,Andrew T. Morse,Edward Nigoghossian,Amanda Nopakun,Linda Okach,Christina Puckett,Ron Reyes,Henry J. Tien,Christine B. Trame,Henry van den Bedem,Dana Weekes,Tiffany Wooten,Andrew Yeh,Jiadong Zhou,Keith O. Hodgson,John Wooley,Marc‐André Elsliger,Ashley M. Deacon,Adam Godzik,Scott A. Lesley,Ian A. Wilson
出处
期刊:Acta crystallographica [Wiley]
卷期号:66 (10): 1297-1305 被引量:29
标识
DOI:10.1107/s1744309110023055
摘要

Membrane-attack complex/perforin (MACPF) proteins are transmembrane pore-forming proteins that are important in both human immunity and the virulence of pathogens. Bacterial MACPFs are found in diverse bacterial species, including most human gut-associated Bacteroides species. The crystal structure of a bacterial MACPF-domain-containing protein BT_3439 (Bth-MACPF) from B. thetaiotaomicron, a predominant member of the mammalian intestinal microbiota, has been determined. Bth-MACPF contains a membrane-attack complex/perforin (MACPF) domain and two novel C-terminal domains that resemble ribonuclease H and interleukin 8, respectively. The entire protein adopts a flat crescent shape, characteristic of other MACPF proteins, that may be important for oligomerization. This Bth-MACPF structure provides new features and insights not observed in two previous MACPF structures. Genomic context analysis infers that Bth-MACPF may be involved in a novel protein-transport or nutrient-uptake system, suggesting an important role for these MACPF proteins, which were likely to have been inherited from eukaryotes via horizontal gene transfer, in the adaptation of commensal bacteria to the host environment.
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