糖蛋白130
分子生物学
生物
选择性拼接
受体
RNA剪接
逆转录酶
白细胞介素-4受体
互补DNA
白细胞介素-21受体
白细胞介素-6受体
信使核糖核酸
白细胞介素
基因
细胞因子
聚合酶链反应
遗传学
核糖核酸
白细胞介素6
作者
Marianna Csilla Holub,Csaba Szalai,A. A. Polgar,Sára Tóth,András Falus
标识
DOI:10.1016/s0165-2478(99)00040-1
摘要
Receptors for interleukin-6 (IL-6) occur in body fluids in soluble form, as well. This is an approx. 50 kDa protein with the ability to bind IL-6. The soluble IL-6 receptor (sIL-6R)/IL-6 complex can attach to membrane anchored gp130, a molecule associated with the signal transduction induced by IL-6 and by other related cytokines. Earlier we described the appearance of sIL-6R in various body fluids of autoimmune patients. In this study using reverse transcriptase polymerase chain reaction (RT-PCR) we isolated and characterised a truncated form of amplified cDNA reverse-transcribed from IL-6 receptor mRNA both from human hepatoma cell line HepG2 and mononuclear cells from inflammatory bowel disease (IBD) patients. Using digestion by Pvu II restriction endonuclease and direct nucleotide sequencing we conclude that alternative splicing is likely involved in generation of sIL-6R. Our further experiments suggest that IL-6 and recombinant sIL-6R themselves do not influence the alternative splicing of IL-6 receptor gene.
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