Characterization of liquid chromatographic chiral separation on chiral crown ether stationary phases

Abstract Crown ether‐based chiral stationary phases (CSPs) have been known to be quite useful for the liquid chromatographic resolution of racemic compounds containing a primary amino group. Chiral separations on crown ether‐based CSPs are characterized by several factors. In this paper, the structural characteristics of five selected crown ether‐based CSPs, analyte characteristics and several factors characterizing chiral separations such as the mobile phase modifiers and the column temperature were reviewed. Among the various factors, the column temperature and the inorganic modifier in the mobile phase influence the chiral separations equally for the five selected crown ether‐based CSPs. In contrast, the effect of the organic and acidic modifier in the aqueous mobile phase on the chiral separations depends on the structural characteristics of CSPs.