流式细胞术
免疫系统
肿瘤微环境
生物
癌症研究
背景(考古学)
肿瘤浸润淋巴细胞
放射治疗
细胞毒性T细胞
免疫学
免疫疗法
病理
体外
医学
内科学
古生物学
生物化学
作者
Sara Labiano,Irantzu Serrano-Mendioroz,María E. Rodriguez-Ruiz
标识
DOI:10.1016/bs.mcb.2022.07.005
摘要
The immunomodulatory properties of local hypofractionated radiotherapy are known to promote the generation of anti-tumor immune responses. Such responses are largely due to the infiltration of cytotoxic lymphocytes (TILs) into the tumors that are able to destroy malignant lesions. In this context, characterizing the tumor immune microenvironment following radiotherapy is crucial for the study of its mechanism of action. Flow cytometry-based analyses are frequently used to elucidate changes in the tumor immune microenvironment. The use of a fluorochrome-conjugated antibody panel is currently a standard technique to assess the number and phenotype of immune cell populations infiltrating the tumors. Here, we describe a method to isolate and quantify TILs based on flow-cytometry in mammary carcinoma-bearing mice that undergo a local hypofractionated radiotherapy regimen consisting of 3 consecutive doses of 8 Gy. With some adaptations, this protocol can be successfully applied to a diverse range of transplantable and inducible solid mouse tumors of different origins.
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