工具箱
计算生物学
纳米技术
计算机科学
生化工程
工程类
程序设计语言
生物
材料科学
作者
Lihua Liu,Yubo Huang,Yufeng Zhou,Yu Zhao,Jinzhi Qi,Xin Zhang,Baoxing Shen
标识
DOI:10.1016/j.trac.2022.116764
摘要
Protein aggregation is a process wherein misfolded proteins accumulate to assemble various forms of oligomeric or aggregated structures. This process has been closely related to a variety of human diseases, primarily represented by neurodegenerative diseases. This review provides an overview of fluorescent methods, in particular fluorogenic approaches, that can detect and follow the multiple steps of protein aggregation both in vitro and in vivo . To emphasize the advantage of these approaches, they are compared with other non-fluorescent methods, including western blotting, spectroscopy, and dynamic light scattering. Furthermore, chemical mechanisms that enable the development of these fluorogenic probes are discussed, with a focus on design principles and physical mechanisms underlying fluorescent signal changes that are induced by the process of protein aggregation. In the end, a perspective of challenges and opportunities are discussed to potentiate future method development to study protein aggregation, as well as related biological and biomedical questions. • A brief introduction of the protein aggregation process • An overview of fluorogenic methods and their parallel comparisons with traditional methods • Designing principles and physical mechanisms of detection process are discussed in detail
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