Rapid and label-free electrochemical aptasensor based on a palladium nanoparticles/titanium carbide/polyethyleneimine functionalized nitrogen-doped carbon nanotubes composite for amplified detection of streptomycin

A novel electrochemical aptasensor for the determination of streptomycin (STR) was constructed in this work. We prepared a palladium nanoparticles/titanium carbide/polyethyleneimine-functionalized nitrogen-doped carbon nanotube (Pd@Ti3C2-PEI-NCNTs) composite via hydrothermal-assisted formic acid reduction and employed it as a substrate material for anchoring a NH2-functionalized aptamer (Apt) to the surface of an electrode through the Pd-N bond. The Pd@Ti3C2-PEI-NCNTs composite increased the electrochemically active surface area and conductivity of the electrode, achieving substantial signal amplification (1.87 times). The constructed aptasensor demonstrated accurate STR detection via specific recognition between the Apt and STR, with a detection range and limit of 0.01-700 and 0.003 nmol/L, respectively. The aptasensor also exhibited excellent selectivity, stability, reproducibility, practicability and shorter assay times (60 min). Furthermore, molecular docking and molecular dynamic also elucidated the binding mechanism between the aptamer and STR molecules. Overall, this method is expected to be a helpful tool for detecting STR in food.