亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Purification and characterization of a glycoprotein from Sipunculus nudus and its immune-enhancing activity to RAW 264.7 macrophages

糖蛋白 生物化学 糖苷键 圆二色性 化学 免疫系统 肽序列 氨基酸 生物 免疫学 基因
作者
Xuli Lu,Yaping Dai,Sheng‐Tao Yang,Tengfei Fu,Yunxia He,Fanke Zeng,Tinghui Chen,Yupo Cao,Ruyi Li,Jihua Li,Wei Zhou
出处
期刊:Food Research International [Elsevier BV]
卷期号:174: 113591-113591 被引量:8
标识
DOI:10.1016/j.foodres.2023.113591
摘要

Sipunculus nudus, an edible marine invertebrate, has long been used as traditional Chinese medicine in folk remedies. In order to assess the immunoregulatory activity of glycoproteins in Sipunculus nudus and conduct a structure–activity relationship, a glycoprotein (SGP1) with molecular mass of 9.26 kDa was purified from Sipunculus nudus, and its chemical structure as well as immune-enhancing activity was investigated in this study. Structure analysis revealed that SGP1, a protein-dominate glycoprotein with O-glycosidic bonds, contained 92.8 % protein and 3.1 % saccharide. GC–MS result indicated that the saccharide moieties of SGP1 basically consisted of lyxose (Lyx), xylose (Xyl) as well as glucose (Glu) at a molar proportion of 0.87:4.16:1.36. The fourier transform infrared specoscopy (FT-IR) result proved that SGP1 have a typical characteristic of glycoprotein. Besides, circular dichroism (CD) result showed that SGP1 contained 4.1 % α-helix, 42.5 % β-sheet, 21.4 % β-turn, and 32.0 % random coil, indicating it's mainly a β-sheet glycoprotein. The amino acid sequence of SGP1 shared a similarity to the Myohemerythrin (sp|Q5K473|HEMTM) with protein sequence coverage of 28.3 %. Moreover, the activity evaluation results showed that SGP1 exhibited significant immune-enhancing activity to the RAW 264.7 macrophages by promoting macrophages proliferation, enhancing phagocytic capacity, and simultaneously stimulating the secretions of nitric oxide (NO), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) via NF-κB pathways. In this study, SGP1 as a novel glycoprotein had an obvious immune-enhancing activity to macrophages, and thus could be applied in the functional foods as a potential immunopotentiator for the hypoimmune population.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
yar应助wen采纳,获得10
1秒前
核桃发布了新的文献求助30
4秒前
迷人问兰发布了新的文献求助10
9秒前
16秒前
牛牛完成签到 ,获得积分10
1分钟前
时间煮雨我煮鱼完成签到,获得积分10
1分钟前
Plum22发布了新的文献求助10
1分钟前
BiuBiu怪完成签到,获得积分10
2分钟前
bkagyin应助陈苗采纳,获得10
2分钟前
核桃发布了新的文献求助10
2分钟前
Plum22完成签到 ,获得积分10
3分钟前
自由觅松发布了新的文献求助20
3分钟前
3分钟前
核桃发布了新的文献求助10
3分钟前
深情安青应助科研通管家采纳,获得10
3分钟前
科研通AI2S应助科研通管家采纳,获得10
3分钟前
阳阳阳发布了新的文献求助10
3分钟前
阳阳阳完成签到,获得积分10
4分钟前
4分钟前
4分钟前
CATH完成签到 ,获得积分10
4分钟前
4分钟前
zhanghao发布了新的文献求助10
4分钟前
Hillson完成签到,获得积分10
4分钟前
糯糯完成签到 ,获得积分10
4分钟前
5分钟前
自由飞阳完成签到,获得积分10
5分钟前
小羡完成签到 ,获得积分10
5分钟前
滕皓轩完成签到 ,获得积分20
5分钟前
aDou完成签到 ,获得积分10
5分钟前
George完成签到,获得积分10
5分钟前
oscar发布了新的文献求助10
5分钟前
oscar完成签到,获得积分10
5分钟前
6分钟前
6分钟前
6分钟前
6分钟前
疯狂的自行车完成签到,获得积分20
7分钟前
Akim应助科研通管家采纳,获得10
7分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990084
求助须知:如何正确求助?哪些是违规求助? 3532108
关于积分的说明 11256447
捐赠科研通 3271016
什么是DOI,文献DOI怎么找? 1805171
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809228