水解
化学
聚对苯二甲酸乙二醇酯
水解酶
突变
降级(电信)
酶
定向进化
生物化学
突变
突变体
计算机科学
材料科学
基因
电信
复合材料
作者
Yunxin Zheng,Jiaxing Zhang,Shengping You,Wei Lin,Rongxin Su,Wei Qi
标识
DOI:10.1016/j.biortech.2024.130929
摘要
Polyethylene terephthalate (PET) has caused significant pollution issues. Compared to chemical degradation with high energy consumption and cost, enzymatic degradation offers a sustainable solution for PET waste recycling. However, the hydrolytic activity of current PET hydrolases still requires improvement. In this study, a cross-correlation-based accumulated mutagenesis (CAM) strategy was developed to enhance the hydrolysis activity. By mitigating epistatic effect and combinational mutations, we achieved a highly active variant LCC-YGA (H183Y/L124G/S29A) with 2.1-fold hydrolytic activity on amorphous PET films of LCC-ICCG. Conformational analysis elucidated how the introduction of distal mutations enhanced activity. The dynamic correlation among different regions facilitated a synergistic effect, enhancing binding pocket flexibility through remote interactions. Totally, this work offers novel insights and methods for PET hydrolases engineering and provides an efficient enzyme for PET degradation and recycling.
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