Hwangdonia lutea sp. nov., isolated from the Haima cold seep, South China Sea

A Gram-stain-negative, orange-yellow, rod-shaped bacterium, designated strain SCSIO 19198 T , was isolated from sediment of the Haima cold seep in the South China Sea, PR China. The strain was aerobic and non-motile. Growth of strain SCSIO 19198 T occurred at pH 7–9 (optimum, pH 7), 15–37 °C (optimum, 25–32 °C) and with 3–8 % (w/v) NaCl (optimum, 3–6 % NaCl). Phylogenetic analyses based on 16S rRNA sequences revealed that strain SCSIO 19198 T belonged to the genus Hwangdonia , having the highest similarity to Hwangdonia seohaensis HD-3 T (98.35 %), followed by Algibacter aquimarinus KYW589 T (95.17 %) and Gelatiniphilus marinus GYP-24 T (94.89 %). The DNA G+C content was 35.92 mol%. The average nucleotide identity value between the genome of strain SCSIO 19198 T and that of H. seohaensis HD-3 T was 88.49 %. The digital DNA–DNA hybridization value between strain SCSIO 19198 T and H. seohaensis HD-3 T was 36 %. The major fatty acids (>10 %) of strain SCSIO 19198 T were iso-C 15 : 0 , iso-C 15 : 1 G, summed feature 3 (C 16 : 1 ω 6 c /C 16 : 1 ω7 c ) and anteiso-C 15 : 0 . MK-6 was the only detected respiratory quinone. The polar lipids of strain SCSIO 19198 T included phosphatidylethanolamine, two aminolipids, glycolipid and two unidentified lipids. The phenotypic, phylogenetic, chemotaxonomic and genomic data clearly suggest that strain SCSIO 19198 T represents a novel species of the genus Hwangdonia , for which the name Hwangdonia lutea sp. nov. is proposed. The type strain is SCSIO 19198 T (=MCCC 1K08674 T =KCTC 102078 T ).