已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Identifying Hair Biomarker Candidates for Alzheimer’s Disease Using Three High Resolution Mass Spectrometry-Based Untargeted Metabolomics Strategies

代谢组学 生物标志物发现 生物标志物 化学 计算生物学 质谱法 蛋白质组学 色谱法 生物化学 生物 基因
作者
Chih‐Wei Chang,Jen-Yi Hsu,Ping-Zu Hsiao,Yuan-Chih Chen,Pao‐Chi Liao
出处
期刊:Journal of the American Society for Mass Spectrometry [American Chemical Society]
卷期号:34 (4): 550-561 被引量:1
标识
DOI:10.1021/jasms.2c00294
摘要

High-resolution mass spectrometry (HRMS)-based untargeted metabolomics strategies have emerged as an effective tool for discovering biomarkers of Alzheimer's disease (AD). There are various HRMS-based untargeted metabolomics strategies for biomarker discovery, including the data-dependent acquisition (DDA) method, the combination of full scan and target MS/MS, and the all ion fragmentation (AIF) method. Hair has emerged as a potential biospecimen for biomarker discovery in clinical research since it might reflect the circulating metabolic profiles over several months, while the analytical performances of the different data acquisition methods for hair biomarker discovery have been rarely investigated. Here, the analytical performances of three data acquisition methods in HRMS-based untargeted metabolomics for hair biomarker discovery were evaluated. The human hair samples from AD patients (N = 23) and cognitively normal individuals (N = 23) were used as an example. The most significant number of discriminatory features was acquired using the full scan (407), which is approximately 10-fold higher than that using the DDA strategy (41) and 11% higher than that using the AIF strategy (366). Only 66% of discriminatory chemicals discovered in the DDA strategy were discriminatory features in the full scan dataset. Moreover, compared to the deconvoluted MS/MS spectra with coeluted and background ions from the AIF method, the MS/MS spectrum obtained from the targeted MS/MS approach is cleaner and purer. Therefore, an untargeted metabolomics strategy combining the full scan with the targeted MS/MS method could obtain most discriminatory features along with a high quality MS/MS spectrum for discovering the AD biomarkers.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
卡皮巴拉完成签到,获得积分10
刚刚
清爽源智完成签到,获得积分10
3秒前
5秒前
漂亮采波完成签到,获得积分10
6秒前
YamDaamCaa应助科研顺利啦采纳,获得50
7秒前
敏感的南露完成签到,获得积分10
9秒前
Nefelibata完成签到,获得积分10
10秒前
bystanding完成签到,获得积分10
10秒前
慕青应助zty123采纳,获得10
12秒前
彪壮的银耳汤完成签到 ,获得积分10
14秒前
轩辕山槐完成签到,获得积分10
20秒前
21秒前
zty123完成签到,获得积分10
23秒前
大树完成签到 ,获得积分10
25秒前
25秒前
干净思远完成签到,获得积分10
25秒前
zty123发布了新的文献求助10
27秒前
领导范儿应助漂亮采波采纳,获得10
28秒前
量子星尘发布了新的文献求助10
28秒前
zxy完成签到 ,获得积分10
28秒前
科研通AI2S应助科研通管家采纳,获得30
29秒前
29秒前
Akim应助科研通管家采纳,获得10
29秒前
29秒前
29秒前
29秒前
29秒前
29秒前
852应助科研通管家采纳,获得10
29秒前
Limerencia完成签到,获得积分10
30秒前
echo完成签到 ,获得积分10
32秒前
33秒前
35秒前
37秒前
土豆你个西红柿完成签到 ,获得积分10
38秒前
Doki发布了新的文献求助10
39秒前
芯之痕发布了新的文献求助10
40秒前
哔噗哔噗完成签到,获得积分10
42秒前
学霸宇大王完成签到 ,获得积分10
43秒前
43秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3976600
求助须知:如何正确求助?哪些是违规求助? 3520674
关于积分的说明 11204422
捐赠科研通 3257298
什么是DOI,文献DOI怎么找? 1798683
邀请新用户注册赠送积分活动 877842
科研通“疑难数据库(出版商)”最低求助积分说明 806595