FOXM1 Participates in Trophoblast Migration and Early Trophoblast Invasion: Potential Role in Blastocyst Implantation

滋养层 胚泡 胎盘形成 男科 生物 基质凝胶 福克斯M1 血管生成 细胞生物学 下调和上调 胎盘 癌症研究 胚胎 胚胎发生 胎儿 医学 怀孕 遗传学 基因
作者
Reyna Peñailillo,Victoria Velásquez,Stephanie Acuña‐Gallardo,Felipe García,Mario Sánchez,Gino Nardocci,Sebastián E. Illanes,Lara J. Monteiro
出处
期刊:International Journal of Molecular Sciences [MDPI AG]
卷期号:25 (3): 1678-1678 被引量:1
标识
DOI:10.3390/ijms25031678
摘要

Successful implantation requires coordinated migration and invasion of trophoblast cells into a receptive endometrium. Reduced forkhead box M1 (FOXM1) expression limits trophoblast migration and angiogenesis in choriocarcinoma cell lines, and in a rat model, placental FOXM1 protein expression was significantly upregulated in the early stages of pregnancy compared to term pregnancy. However, the precise role of FOXM1 in implantation events remains unknown. By analyzing mice blastocysts at embryonic day (E3.5), we have demonstrated that FOXM1 is expressed as early as the blastocyst stage, and it is expressed in the trophectoderm of the blastocyst. Since controlled oxygen tension is determinant for achieving normal implantation and placentation and a chronic hypoxic environment leads to shallow trophoblast invasion, we evaluated if FOXM1 expression changes in response to different oxygen tensions in the HTR-8/SVneo first trimester human trophoblast cell line and observed that FOXM1 expression was significantly higher when trophoblast cells were cultured at 3% O2, which coincides with oxygen concentrations in the uteroplacental interface at the time of implantation. Conversely, FOXM1 expression diminished in response to 1% O2 that resembles a hypoxic environment in utero. Migration and angiogenesis were assessed following FOXM1 knockdown and overexpression at 3% O2 and 1% O2, respectively, in HTR-8/SVneo cells. FOXM1 overexpression increased transmigration ability and tubule formation. Using a 3D trophoblast invasion model with trophospheres from HTR-8/SVneo cells cultured on a layer of MATRIGEL and of mesenchymal stem cells isolated from menstrual fluid, we observed that trophospheres obtained from 3D trophoblast invasion displayed higher FOXM1 expression compared with pre-invasion trophospheres. Moreover, we have also observed that FOXM1-overexpressing trophospheres increased trophoblast invasion compared with controls. HTR-8/SVneo-FOXM1-depleted cells led to a downregulation of PLK4, VEGF, and MMP2 mRNA expression. Our current findings suggest that FOXM1 participates in embryo implantation by contributing to trophoblast migration and early trophoblast invasion, by inducing transcription activation of genes involved in these processes. Maternal-fetal communication is crucial for trophoblast invasion, and maternal stromal cells may induce higher levels of FOXM1 in trophoblast cells.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
英俊的铭应助甜甜圈采纳,获得10
刚刚
小点点发布了新的文献求助10
刚刚
1秒前
1秒前
1秒前
柚子发布了新的文献求助10
2秒前
2秒前
3秒前
4秒前
翻译度完成签到,获得积分10
4秒前
星河zp发布了新的文献求助10
5秒前
研友_LwlAgn发布了新的文献求助10
6秒前
msl2023完成签到,获得积分10
7秒前
打打应助甜味拾荒者采纳,获得10
7秒前
miao发布了新的文献求助10
8秒前
YJ888发布了新的文献求助10
8秒前
9秒前
9秒前
10秒前
小田完成签到,获得积分10
10秒前
柚子完成签到,获得积分10
11秒前
陶醉觅夏发布了新的文献求助10
12秒前
甜甜圈发布了新的文献求助10
14秒前
ding应助小研采纳,获得10
14秒前
CodeCraft应助汤飞柏采纳,获得10
15秒前
Ava应助研友_LwlAgn采纳,获得10
17秒前
17秒前
华仔应助jingmishensi采纳,获得10
18秒前
脱壳金蝉完成签到,获得积分10
18秒前
呜呜呜完成签到,获得积分10
19秒前
迷路中的骑手完成签到,获得积分10
20秒前
斯文败类应助飞飞飞采纳,获得10
21秒前
21秒前
qiaoj2006完成签到,获得积分10
22秒前
我是老大应助Eskimo采纳,获得10
22秒前
22秒前
所所应助简单起眸采纳,获得10
22秒前
哆啦顺利毕业完成签到 ,获得积分10
23秒前
intangible完成签到,获得积分10
24秒前
星辰大海应助科研通管家采纳,获得10
24秒前
高分求助中
Licensing Deals in Pharmaceuticals 2019-2024 3000
Cognitive Paradigms in Knowledge Organisation 2000
Effect of reactor temperature on FCC yield 2000
Introduction to Spectroscopic Ellipsometry of Thin Film Materials Instrumentation, Data Analysis, and Applications 1800
Natural History of Mantodea 螳螂的自然史 1000
A Photographic Guide to Mantis of China 常见螳螂野外识别手册 800
How Maoism Was Made: Reconstructing China, 1949-1965 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3313419
求助须知:如何正确求助?哪些是违规求助? 2945813
关于积分的说明 8527122
捐赠科研通 2621489
什么是DOI,文献DOI怎么找? 1433679
科研通“疑难数据库(出版商)”最低求助积分说明 665080
邀请新用户注册赠送积分活动 650600