血小板源性生长因子受体
刺激
下调和上调
成纤维细胞
化学
肌成纤维细胞
血小板衍生生长因子
细胞生物学
Ⅰ型胶原
成纤维细胞生长因子受体1
生长因子
成纤维细胞生长因子
受体
内科学
内分泌学
生物
医学
基因
体外
生物化学
纤维化
作者
Hiroya Urabe,Ryuji Akimoto,Shohei Kamiya,Katsu Hosoki,Hideyuki Ichikawa,Toshio Nishiyama
摘要
ABSTRACT Pulsed electrical stimulation (PES) is known to affect cellular activities. We previously found PES to human dermal fibroblasts (HFs) promoted platelet-derived growth factor subunit A (PDGFA) gene expression, which enhanced proliferation. In this study, we investigated PES effects on fibroblast collagen production and differentiation into myofibroblasts. HFs were electrically stimulated at 4800 Hz and 5 V for 60 min. Imatinib, a specific inhibitor of PDGF receptors, was treated before PES. After 6 h of PES, PDGFA, α-smooth muscle actin (α-SMA), and collagen type I α1 chain gene expressions were upregulated in PES group. Imatinib suppressed the promoted expression except for PDGFA. Immunofluorescence staining and enzyme-linked immunosorbent assay showed the production of α-SMA and collagen I was enhanced in PES group but suppressed in PES + imatinib group at 48 h after PES. Therefore, PES promotes the production of α-SMA and collagen I in fibroblasts, which is triggered by PDGFA that is upregulated early after PES.
科研通智能强力驱动
Strongly Powered by AbleSci AI