黄曲霉毒素
色谱法
检出限
适体
化学
高效液相色谱法
衍生化
亲和层析
残留物(化学)
最大残留限量
农药残留
杀虫剂
食品科学
生物
生物化学
遗传学
酶
农学
作者
Cong Ji,Xinyang Sun,Yong Fang,Peng Li
出处
期刊:Foods
[MDPI AG]
日期:2024-02-20
卷期号:13 (5): 640-640
被引量:1
标识
DOI:10.3390/foods13050640
摘要
Aflatoxin B1 (AFB1) is a highly teratogenic and carcinogenic secondary metabolite produced by Aspergillus. It is commonly detected in agricultural products such as cereals, peanuts, corn, and feed. Grains have a complex composition. These complex components severely interfere with the effective extraction and separation of AFB1, and also cause problems such as matrix interference and instrument damage, thus posing a great challenge in the accurate analysis of AFB1. In this study, an aptamer affinity column for AFB1 analysis (AFB1-AAC) was prepared for the enrichment and purification of AFB1 from grain samples. AFB1-AAC with an AFB1-specific aptamer as the recognition element exhibited high affinity and specificity for AFB1. Grain samples were enriched and purified by AFB1-AAC, and subsequently analyzed by high performance liquid chromatography with post-column photochemical derivatization-fluorescence detection (HPLC-PCD-FLD). The average recoveries of AFB1 ranged from 88.7% to 99.1%, with relative standard deviations (RSDs) of 1.4–5.6% (n = 3) at the spiked levels of 5.0–20.0 μg kg−1. The limit of detection (LOD) for AFB1 (0.02 μg kg−1) was much below the maximum residue limits (MRLs) for AFB1. This novel method can be applied to the determination of AFB1 residues in peanut, corn, and rice.
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