Transcriptomic analysis of ovarian follicles uncovers the crucial genes relevant to follicle selection and preovulatory hierarchy in hens

转录组 生物 毛囊 基因 卵泡 细胞生物学 信号转导 内科学 卵巢 内分泌学 基因表达 遗传学 医学
作者
Xue Sun,Hongyan Zhu,Changyan Zhang,Jean Philippe Harold Wensesso Ilboudo,Jinghua Zhao,Chang Ma,Chunchi Yan,Simushi Liswaniso,Ning Qin,Rifu Xu
出处
期刊:Journal of Animal Science [Oxford University Press]
卷期号:101
标识
DOI:10.1093/jas/skad241
摘要

Abstract Follicle selection and preovulatory hierarchy of hen ovaries were important stages of follicle development and crucially determining egg-laying performance. The selected follicles with a higher expression level of follicle-stimulating hormone receptor (FSHR) mRNA that facilitates response to FSH, and rapidly develops into preovulatory follicles with distinctive characteristics of granulosa cells (GCs) proliferation and differentiation. Identification of the key genes involved in these developmental events is helpful for elucidation of the molecular mechanism underlying egg-laying traits in chicken and other domestic fowl. Herein, the comparative transcriptomic analysis of ovarian prehierarchical follicles before selection (BSF), follicles at selection stage (ASF), and hierarchical follicles (HF) were implemented in the Jilin Black chicken (JB) and Lohmann Brown layer (LB) with the divergences in their egg-laying performance by RNA-sequencing. The results showed that nine deferentially expressed genes (DEGs), including STMN4, FABP3, ROBO2, RSPO4, and DMRT1 were revealed between follicles BSF and ASF; and seventeen DEGs, such as SLC6A15, SLITRK3, PRKG2 and TMC3 were mined between ASF and HF. These two group DEGs being co-expressed between BSF and ASF, and between ASF and HF were compared and substantiated in the JB and LB layers, respectively. Furthermore, 10 signaling pathways, such as cAMP signaling, PPAR signaling pathway, AMPK(Adenosine 5'-monophosphate (AMP)-activated protein kinase) pathway, and estrogen signaling pathway were also identified. Moreover, the roles of two representative candidates ROBO2 and PRKG2 genes presented as downregulated mRNA expression pattern in the transcriptomic profiles were further verified in vitro. The results demonstrated that downregulation of ROBO2 or PRKG2 significantly increased the expression levels of FSHR mRNA and protein with the boosted expression of CCND1, STAR, and BCL-2, whereas remarkably inhibited the expression of Caspase-3, consequently, brought about the decrease of GC apoptosis in the ovarian follicles, but increase of GC proliferation and differentiation serving as the hallmarks for follicle selection. It indicated that ROBO2 and PRKG2 may play indispensable roles in follicle selection and preovulatory hierarchy of hen ovaries separately. Our findings provided a comparative transcriptomic evidence for clarifying the molecular mechanism of the follicle development underlying egg-laying traits in chicken.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
是danoo发布了新的文献求助10
刚刚
量子星尘发布了新的文献求助30
4秒前
活力妙芙完成签到 ,获得积分10
4秒前
serapy完成签到,获得积分10
4秒前
5秒前
大个应助焦恩俊采纳,获得10
6秒前
酷波er应助执着皮皮虾采纳,获得10
6秒前
小叶子发布了新的文献求助10
6秒前
6秒前
大个应助liuliu采纳,获得10
7秒前
7秒前
11完成签到,获得积分10
8秒前
8秒前
9秒前
今夜有雨完成签到 ,获得积分10
10秒前
10秒前
桐桐应助根深者叶茂采纳,获得10
10秒前
ballball233发布了新的文献求助10
11秒前
NexusExplorer应助科研通管家采纳,获得30
11秒前
kingwill发布了新的文献求助30
11秒前
克劳修斯发布了新的文献求助10
12秒前
上官若男应助科研通管家采纳,获得10
12秒前
12秒前
12秒前
科研通AI5应助科研通管家采纳,获得10
12秒前
orixero应助科研通管家采纳,获得10
12秒前
FashionBoy应助科研通管家采纳,获得100
12秒前
浮游应助科研通管家采纳,获得10
12秒前
科研通AI2S应助科研通管家采纳,获得10
13秒前
赘婿应助科研通管家采纳,获得10
13秒前
浮游应助科研通管家采纳,获得10
13秒前
blue完成签到,获得积分10
13秒前
研友_Z6Qrbn发布了新的文献求助10
15秒前
Panda完成签到,获得积分10
16秒前
16秒前
19秒前
天空之下发布了新的文献求助10
19秒前
19秒前
小豆芽完成签到,获得积分10
19秒前
无花果应助zxd采纳,获得10
19秒前
高分求助中
Comprehensive Toxicology Fourth Edition 24000
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Pipeline and riser loss of containment 2001 - 2020 (PARLOC 2020) 1000
World Nuclear Fuel Report: Global Scenarios for Demand and Supply Availability 2025-2040 800
The Social Work Ethics Casebook(2nd,Frederic G. R) 600
Handbook of Social and Emotional Learning 500
HEAT TRANSFER EQUIPMENT DESIGN Advanced Study Institute Book 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 内科学 生物化学 物理 计算机科学 纳米技术 遗传学 基因 复合材料 化学工程 物理化学 病理 催化作用 免疫学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 5114705
求助须知:如何正确求助?哪些是违规求助? 4321984
关于积分的说明 13467476
捐赠科研通 4153626
什么是DOI,文献DOI怎么找? 2275948
邀请新用户注册赠送积分活动 1277982
关于科研通互助平台的介绍 1215920