巴比妥酸
生物分析
人血浆
有机阴离子转运蛋白1
化学
药理学
药品
运输机
药物开发
色谱法
色氨酸
生物化学
医学
氨基酸
基因
作者
Renmeng Liu,Xiaofeng Zhao,Guoju Geng,Yurong Lai
出处
期刊:Bioanalysis
[Newlands Press Ltd]
日期:2022-10-01
卷期号:14 (20): 1327-1336
被引量:1
标识
DOI:10.4155/bio-2022-0177
摘要
Background: Monitoring levels of endogenous biomarkers has become an alternative approach to assess transporter-mediated drug-drug interactions in clinical trials. Among the biomarkers of interest, kynurenic acid is effective for the human organic anion transporters OAT1 and OAT3. Here, a simple and robust bioanalytical method was developed using LC-MS/MS to quantify kynurenic acid in human plasma. Results: This method achieved a LLOQ of 10 nm with acceptable signal-to-noise ratio (S/N >5). In addition, an interfering agent, tryptophan, was identified and separated chromatographically. A full method validation was performed in the spirit of GLP. Conclusion: This method can serve as a tool readily available to assess potential drug-drug interactions mediated by inhibition of OAT1 and OAT3 activities.
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