Analysis of Macrophage Migration Inhibitory Factor Genotype in Hemophilia A Patients

Background Hemophilia A, an X-linked bleeding disorder, is caused by a complete or partial deficiency in Factor VIII. Multiple factors play a role in the development and progression of bleeding episodes, especially arthropathy, in hemophilia patients. Objectives Detection of macrophage migration inhibitory factor (MIF)-173 G/C polymorphism in people with hemophilia A (PWH) and the possible associations between the type of MIF gene polymorphism and selected disease-related variables. Subjects and Methods This case–control study included 95 male patients aged 2 months to 63 years with hemophilia A and 95 nonhemophiliac subjects matched for age and sex. Allele-specific polymerase chain reaction (AS-PCR) with multiplex technique was used to detect MIF polymorphisms. Results A significantly higher frequency of GG polymorphism was reported in the control group (81, 85.3%) compared to PWH (64, 67.4%), while a significantly higher frequency of GC polymorphism was found in PWH (21, 22.1%) than in healthy subjects (10, 10.5%), P0.05. Conclusions MIF-173 GC polymorphism is associated with PWH more than healthy individuals. Further studies are required to detect more SNPs through sequencing of MIF gene and to detect MIF serum levels during bleeding episodes.