Milk clotting enzymes from marine resources and their role in cheese-making: A mini review

With the continual increase in global cheese consumption, rennet, the traditional milk coagulant, is unable to meet the growing demand in cheese production. Although several proteases from other sources have been used for cheese-making, they suffer various shortcomings. The ocean is home to a huge and diverse range of life forms, which represent a vast potential source of proteases. Marine proteases have been isolated from a number of marine species, including sponge, jellyfish, seaweed and marine animals, and some have been shown to be suitable as milk-clotting enzymes for cheese making. This review summarizes the latest studies on rennet substitutes from marine resources and their role in cheese-making. The emphasis of the review is on the isolation and purification of the marine proteases, the biochemical characteristics of these enzymes, especially their caseinolytic and milk-clotting properties, as well as their cleavage sites on casein. Some of the marine proteases have been applied as milk-clotting agent in cheese-making, with the resultant production of cheese with comparable characteristics, including sensory characteristics, to calf rennet cheese. The review concludes by highlighting the challenges and opportunities for future research in the field.