互补DNA
生物
生物化学
甘氨酸
大豆油
基因
基因表达
核糖核酸
油酸
氨基酸
食品科学
作者
Aijing Zhang,Yue Li,Lixue Wang,Sheng Wang,Yixuan Liu,Xinchao Luan,Shuying Liu,Jun Zhang,Huijing Liu,Dan Yao
标识
DOI:10.1021/acs.jafc.3c00993
摘要
Soybean [Glycine max (Linn.) Merr.] is an important oil crop. Long noncoding RNAs (lncRNAs) play a variety of functions in plants. However, their function in the soybean oil synthesis pathway is yet to be uncovered. Here, the lncRNA43234 gene related to soybean oil synthesis was screened, and the full-length cDNA sequence of the lncRNA was obtained using rapid amplification of cDNA ends. Overexpression of lncRNA43234 increased the content of crude protein in seeds, decreased the content of oleic acid, and affected the content of alanine and arginine in free amino acids. RNA interference of the lncRNA43234 gene decreased the crude protein content in seeds. Quantitative real-time polymerase chain reaction analysis revealed that lncRNA43234 influenced the expression of XM_014775786.1 associated with phosphatidylinositol metabolism by acting as a decoy for miRNA10420, thereby affecting the content of soybean oil. Our results provide insights into how lncRNA-mediated competing endogenous RNA regulatory networks are involved in soybean oil synthesis.
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