Consolidated sustainable organic nanozyme integrated with Point-Of-Use sensing platform for dual agricultural and biological molecule detection

The newly introduced organic nanozymes offer a promising alternative to inorganic nanozymes by sustainable and cost-effective solutions. Given the potential of nanozyme-based Point-of-Care (PoC) systems for on-demand molecule sensing; these user-friendly Point-of-Use(PoU) platforms can be highly beneficial for real-world agricultural and biological applications. Herein, a novel, fully organic compound/monomer-based, sustainable organic nanozyme is introduced, that exhibits peroxidase-like enzyme-like catalytic activity. Utilizing a unique self-assembled particle fabrication process, homogenous nanozyme are fabricated within a short time (within 50 nm, D90), exhibiting decent kinetic profiles (Km= 0.046 mM, H2O2), and degradability for improved waste management after its use. OM nanozyme-incorporated colorimetric sensing platforms successfully detect popular agricultural toxic biomolecules and biological molecules while achieving decent molecule sensing performance (practical LOD, glyphosate: at least 1pgmL-1, glucose: 3.47 ngmL-1) with high analytical sensitivity and selectivity. Additionally, a smartphone image-based polynomial regression analysis system and paper-microfluidic hardware were developed to facilitate PoU applications in a lab-free environment with satisfactory analytic sensitivity ( at least 1.6μgmL-1, for glyphosate, and glucose). This sustainable OM nanozyme, coupled with the PoU platform, is envisioned to have broad applications in agricultural and biological systems.