糖尿病性心肌病
肌膜
糖尿病
细胞外基质
心肌细胞
肌节
内科学
细胞生物学
心肌
医学
细胞内
细胞外
肌肉僵硬
细胞生理学
细胞
内分泌学
心肌病
心脏病学
化学
生物
刚度
心力衰竭
生物化学
材料科学
复合材料
作者
G. Romanelli,Lihuén Villarreal,Camila Espasandín,Juan Claudio Benech
出处
期刊:American Journal of Physiology-cell Physiology
[American Physiological Society]
日期:2024-10-07
标识
DOI:10.1152/ajpcell.00273.2024
摘要
Several studies have demonstrated that Diabetes mellitus can increase the risk of cardiovascular disease and remains the principal cause of death in these patients. Costameres connect the sarcolemma with the cytoskeleton and extracellular matrix, facilitating the transmission of mechanical forces and cell signaling. They are related to cardiac physiology because individual cardiac cells are connected by intercalated discs that synchronize muscle contraction. Diabetes impacts the nano-mechanical properties of cardiomyocytes, resulting in increased cellular and left ventricular stiffness, as evidenced in clinical studies of these patients. The question of whether costameric proteins are affected by diabetes in the heart has not been studied. This work analyzes whether T1DM modifies the costameric proteins and coincidentally changes the cellular mechanics in the same cardiomyocytes. The samples were analyzed by immunotechniques using laser confocal microscopy. Significant statistical differences were found in the spatial arrangement of the costameric proteins. However, these differences are not due to their expression. Atomic force microscopy was used to compare intrinsic cellular stiffness between diabetic and normal cardiomyocytes and obtain the first elasticity map sections of diabetic living cardiomyocytes. Data obtained demonstrated that diabetic cardiomyocytes had higher stiffness than control. The present work shows experimental evidence that intracellular changes related to cell-cell and cell-extracellular matrix communication occur, which could be related to cardiac pathogenic mechanisms. These changes could contribute to alterations in the mechanical and electrical properties of cardiomyocytes and consequently, to diabetic cardiomyopathy.
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