染色质
生物
遗传学
基因
近交系
人口
基因组
茉莉酸
计算生物学
人口学
社会学
作者
Yongli Zhu,H.W. Ngan,Tao Zhu,Lili Nan,Wenqiang Li,Yingjie Xiao,Lin Zhuo,Dijun Chen,Xiaoyu Tu,Kang Gao,Jianbing Yan,Silin Zhong,Ning Yang
标识
DOI:10.1101/2024.10.14.618191
摘要
Characterizing the noncoding regions of the genome, particularly the cis-regulatory elements (CREs) located in accessible chromatin regions (ACRs) in gene promoters, is crucial for understanding how gene expression is regulated and how genotype contributes to phenotypic diversity in plants.In this study, we used ATAC-seq to resequence and map the ACRs of 214 maize inbred lines. We identified 82,174 ACRs and reported that 39.55% of them exhibited significant variation across the population. Next, we used the accessibility of ACRs as a quantitative feature and performed a chromatin associability GWAS (caGWAS), resulting in 27,004 caQTLs. Among them, 2,463 were predicted to disrupt the cis-regulatory elements that TF binds in these ACRs, suggesting that TF binding affects chromatin accessibility. Specifically, we identified two caACRs that regulate the expression of fad7, which encodes a fatty acid desaturase and affects the linolenic acid content.Our findings highlight the dynamic nature of maize ACRs and demonstrate that chromatin accessibility information could be used for GWASs, offering key insights into the genetic and regulatory mechanisms of chromatin accessibility and its impact on complex traits in plants.
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