叶柄(昆虫解剖学)
老茧
外植体培养
腋芽
组织培养
苍术
园艺
植物
生物
体外
医学
生物化学
膜翅目
替代医学
中医药
病理
作者
Yuqiu Zhu,Guohua Xia,Hui-Gang Fang,Shun-Hua Fu,HE Fu-ji
出处
期刊:PubMed
日期:2006-03-01
卷期号:29 (3): 212-3
被引量:3
摘要
A study was conducted by tissue culture on the explant of Atractylodes macrocephala Koidz.. The result showed that NAA was a leading factor for induction of callus from leaf blades and the petiole as well as bud differentiation. MS + BA 1.0 mg/L + NAA 0.3 mg/L + GA3 0.2 mg/L was an optimal medium for induction of callus from leaf blades and the petiole and for the bud differentation also. MS + BA 3.0 mg/L + IBA 0.5 mg/L was suitable for the proliferation of axillary buds, with an induction rate of 95%. 1/2MS + IBA 0.1 - 0.5 mg/L was optimum for rooting, with a rooting percentage more than 90%. The survival rate of transplanted plantlets was more than 90%.
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