The immunogenicity of soluble haptenated polymers is determined by molecular mass and hapten valence.

半抗原 聚合物 菲科尔 聚丙烯酰胺 聚乙烯醇 化学 羧甲基纤维素 右旋糖酐 分子质量 免疫原性 高分子化学 色谱法 体外 有机化学 抗体 生物化学 免疫学 生物 外周血单个核细胞
作者
Renée Z. Dintzis,Masako Okajima,M H Middleton,Gretchen Greene,Howard M. Dintzis
出处
期刊:Journal of Immunology [The American Association of Immunologists]
卷期号:143 (4): 1239-1244 被引量:160
标识
DOI:10.4049/jimmunol.143.4.1239
摘要

T cell-independent Ag are believed to stimulate antibody formation in the relative absence of Ag processing and T cell help. Previous studies on the type 2 T cell independent (TI-2) Ag DNP-polyacrylamide, have shown that when one systematically varies the molecular mass and hapten valence, the immunogenic potential of this type of molecule depends on definable molecular characteristics. It was found that to be immunogenic, these molecules had to exceed a threshold molecular mass of 100,000 Da and a threshold hapten valence of 20. The present study was undertaken to determine whether such findings could be generalized to other molecules belonging to the TI-2 class of Ag. The molecular characteristics of five chemically different fluoresceinated (FL)-polymers were systematically varied, and their ability to stimulate an IgM antihapten immune response was measured. The polymers used as carriers were carefully size-fractionated and consisted of one natural polymer (dextran), one modified natural polymer (carboxymethyl cellulose), and three synthetic polymers (Ficoll, polyvinyl alcohol, and polyacrylamide). The carriers varied in physical structure from the highly cross-linked Ficoll, to the somewhat branched dextran, to the linear polyacrylamide, carboxymethyl cellulose, and polyvinyl alcohol. Polymers were haptenated with FL and size-fractionated so as to yield a panel of molecules with varying molecular mass, hapten valence, and hapten density. Anti-FL IgM response to these haptenated polymers was measured in vivo after i.p. injection of the FL-polymer in saline, and measured in vitro after culture with unfractionated spleen cells from naive mice. In agreement with the previous studies on DNP-polyacrylamide, it was found that to be immunogenic, each of the FL-polymers had to exceed a comparable threshold value of molecular mass and of hapten valence. Optimal immunogenicity occurred when the FL-polymers had values of mass and hapten density lying within a predictable range. Immunogenicity decreased when these optimal parameters were substantially increased or decreased. We conclude that the immunogenicity of soluble haptenated polymers depends on predictable physical molecular characteristics, and is relatively independent of the chemical composition and conformation of the carrier polymer.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
蓝桉发布了新的文献求助10
1秒前
田様应助Boooooo采纳,获得10
2秒前
天之道发布了新的文献求助10
2秒前
拉拉应助linglingling采纳,获得10
2秒前
Vincent完成签到,获得积分10
2秒前
寻水的鱼发布了新的文献求助10
3秒前
yimingzhangbp发布了新的文献求助20
4秒前
4秒前
CipherSage应助bckl888采纳,获得30
4秒前
4秒前
sb发布了新的文献求助10
4秒前
don完成签到,获得积分20
5秒前
6秒前
7秒前
7秒前
研友_xnE4XL完成签到,获得积分10
7秒前
小溪发布了新的文献求助10
7秒前
华仔应助zhgj采纳,获得10
9秒前
10秒前
赘婿应助直率的彤采纳,获得10
11秒前
vermouth发布了新的文献求助10
11秒前
uno发布了新的文献求助10
11秒前
11秒前
11秒前
科研通AI2S应助东郭依风采纳,获得10
11秒前
yy给yy的求助进行了留言
11秒前
believe完成签到,获得积分10
12秒前
英姑应助lty采纳,获得10
12秒前
李爱国应助jackycas采纳,获得10
12秒前
12秒前
13秒前
Kru发布了新的文献求助30
14秒前
14秒前
14秒前
wilaken完成签到,获得积分20
15秒前
15秒前
寻水的鱼完成签到,获得积分10
15秒前
科研通AI2S应助冷傲半烟采纳,获得30
16秒前
16秒前
16秒前
高分求助中
좌파는 어떻게 좌파가 됐나:한국 급진노동운동의 형성과 궤적 2500
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
CLSI EP47 Evaluation of Reagent Carryover Effects on Test Results, 1st Edition 800
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
Retention of title in secured transactions law from a creditor's perspective: A comparative analysis of selected (non-)functional approaches 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3054545
求助须知:如何正确求助?哪些是违规求助? 2711512
关于积分的说明 7426610
捐赠科研通 2356104
什么是DOI,文献DOI怎么找? 1247642
科研通“疑难数据库(出版商)”最低求助积分说明 606478
版权声明 596079