基因敲除
细胞生物学
转录因子
生物
磷酸化
刺激
细胞培养
抄写(语言学)
分子生物学
化学
基因
遗传学
内分泌学
语言学
哲学
作者
Lingyu Hu,Weiwei Zhang,Zheng Xiang,Yali Wang,Cheng Zeng,Xiaojie Wang,Chengning Tan,Yichi Zhang,Fengjie Li,Yanni Xiao,Luping Zhou,Jiuxuan Li,Chun Wu,Yang Xiang,Lan Xiang,Xiaomei Zhang,Xueying Wang,Wenzhong Yang,Maoshan Chen,Qian Ran,Zhongjun Li,Li Chen
出处
期刊:Platelets
[Informa]
日期:2021-10-26
卷期号:33 (5): 755-763
标识
DOI:10.1080/09537104.2021.1988548
摘要
Megakaryocytes (MKs) are the unique non-pathological cells that undergo polyploidization in mammals. The polyploid formation is critical for understanding the MK biology, and transcriptional regulation is involved in the differentiation and maturation of MKs. However, little is known about the functions of transcriptional elongation factors in the MK polyploidization. In this study, we investigated the role of transcription elongation factor EloA in the polyploidy formation during the MK differentiation. We found that EloA was highly expressed in the erythroleukemia cell lines HEL and K562. Knockdown of EloA in HEL cell line was shown to impair the phorbol myristate acetate (PMA) induced polyploidization process, which was used extensively to model megakaryocytic differentiation. Selective over-expression of EloA mutants with Pol II elongation activity partially restored the polyploidization. RNA-sequencing revealed that knockdown of EloA decelerated the transcription of genes enriched in the ERK1/2 cascade pathway. The phosphorylation activity of ERK1/2 decreased upon the EloA inhibition, and the polyploidization process of HEL was hindered when ERK1/2 phosphorylation was inhibited by PD0325901 or SCH772984. This study evidenced a positive role of EloA in HEL polyploidization upon PMA stimulation through enhanced ERK1/2 activity.
科研通智能强力驱动
Strongly Powered by AbleSci AI