大肠杆菌
补料分批培养
重组DNA
批处理
生物量(生态学)
质粒
工艺工程
下游加工
发酵
过程(计算)
计算机科学
化学
生化工程
生物技术
生物系统
生物
食品科学
工程类
色谱法
生物化学
DNA
基因
农学
程序设计语言
操作系统
作者
Thomas P. Caldwell,Benjamin F. Synoground,Sarah W. Harcum
出处
期刊:Methods in Enzymology
日期:2021-01-01
卷期号:: 189-217
被引量:1
标识
DOI:10.1016/bs.mie.2021.05.004
摘要
Fed-batch processes are commonly used in industry to obtain sufficient biomass and associated recombinant protein or plasmids. In research laboratories, it is more common to use batch cultures, as the setup of fed-batch processes can be challenging. This method outlines a robust and reliable means to generate Escherichia coli biomass in a minimum amount of fermentation time using a standardized fed-batch process. Final cell densities can reach over 50g dry cell weight per liter (gdcw/L) depending on the strain. This method uses a predefined exponential feeding strategy and conservative induction protocol to achieve these targets without multiple trial and error studies. If desired, productivity can be optimized by balancing the induction time and feed rates. This method utilizes cost-efficient defined media, minimizes process control complexity, and potentially aids downstream purification.
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