光老化
人体皮肤
离体
真皮
表皮(动物学)
皮肤老化
弹性蛋白
光防护
化学
皮肤当量
皮肤癌
体内
医学
紫外线a
皮肤病科
经皮失水
氧化应激
红斑
活性氧
病理
角质形成细胞
体外
生物
解剖
生物化学
生物技术
遗传学
作者
Bianca Oliveira Saguie,Rayssa Lopes Martins,Adenilson de Souza da Fonseca,Bruna Romana‐Souza,Andréa Monte‐Alto‐Costa
标识
DOI:10.1016/j.jphotobiol.2021.112255
摘要
Skin is the largest body organ and can be affected by several factors, such as ultraviolet (UV) radiation. UV radiation is subdivided in UVA, UVB and UVC according to the radiation wavelength. UVC radiation does not cross the ozone layer; UVB cause DNA damage and is closely related to carcinogenesis; UVA radiation penetrates deeply into the skin, reaching epidermis and dermis and is considered the main promoter of skin aging, known as photoaging. In order to understand photoaging mechanisms and propose efficient therapies, several photoaging study models have been developed, each with benefits and limitations, but most of them use very high doses of UVA radiation, which is not compatible with our daily sun exposure. The objective of this work was to develop a human ex vivo photoaging model induced by UVA exposure compatible to a summer in Brazil. For this, human skin fragments were obtained from healthy donors who underwent otoplasty surgery and skin explants were prepared and placed in plates, with the epidermis facing upwards. Skin explants were exposed to UVA at 16 J/cm2 carried out by protocols of 2 or 4 exposures. Results showed an increase of oxidative damage, inflammatory cells, collagenolytic and elastolytic MMPs expression as well as a decrease of elastin expression, suggesting that the experimental model based on skin explants is able to evaluate UVA-induced aging in human skin.
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