The development of autoverification system of lymphocyte subset assays on the flow cytometry platform

淋巴细胞 流式细胞术 淋巴细胞亚群 免疫学 T淋巴细胞 细胞仪 人类免疫缺陷病毒(HIV) 免疫系统 医学 生物 T细胞
作者
Jue Shi,Runqing Mu,Pan Wang,Wenqing Geng,Yongjun Jiang,Min Zhao,Hong Shang,Zining Zhang
出处
期刊:Clinical Chemistry and Laboratory Medicine [De Gruyter]
卷期号:60 (1): 92-100 被引量:2
标识
DOI:10.1515/cclm-2021-0736
摘要

Abstract Objectives Peripheral blood lymphocyte subsets are important parameters for monitoring immune status; however, lymphocyte subset detection is time-consuming and error-prone. This study aimed to explore a highly efficient and clinically useful autoverification system for lymphocyte subset assays performed on the flow cytometry platform. Methods A total of 94,402 lymphocyte subset test results were collected. To establish the limited-range rules, 80,427 results were first used (69,135 T lymphocyte subset tests and 11,292 NK, B, T lymphocyte tests), of which 15,000 T lymphocyte subset tests from human immunodeficiency virus (HIV) infected patients were used to set customized limited-range rules for HIV infected patients. Subsequently, 13,975 results were used for historical data validation and online test validation. Results Three key autoverification rules were established, including limited-range, delta-check, and logical rules. Guidelines for addressing the issues that trigger these rules were summarized. The historical data during the validation phase showed that the total autoverification passing rate of lymphocyte subset assays was 69.65% (6,941/9,966), with a 67.93% (5,268/7,755) passing rate for T lymphocyte subset tests and 75.67% (1,673/2,211) for NK, B, T lymphocyte tests. For online test validation, the total autoverification passing rate was 75.26% (3,017/4,009), with 73.23% (2,191/2,992) for the T lymphocyte subset test and 81.22% (826/1,017) for the NK, B, T lymphocyte test. The turnaround time (TAT) was reduced from 228 to 167 min using the autoverification system. Conclusions The autoverification system based on the laboratory information system for lymphocyte subset assays reduced TAT and the number of error reports and helped in the identification of abnormal cell populations that may offer clues for clinical interventions.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Orange应助guanghuawang采纳,获得10
刚刚
zhou完成签到,获得积分10
刚刚
kuoping完成签到,获得积分0
1秒前
2秒前
chen发布了新的文献求助10
2秒前
lelele发布了新的文献求助10
2秒前
科研通AI5应助Hexagram采纳,获得10
3秒前
3秒前
深情安青应助道松先生采纳,获得10
3秒前
KSDalton完成签到,获得积分10
3秒前
3秒前
tuanzi发布了新的文献求助10
4秒前
5秒前
pcr163应助仿生人采纳,获得100
6秒前
6秒前
xrL完成签到,获得积分10
6秒前
8秒前
HonamC完成签到,获得积分10
8秒前
星辰大海应助肯瑞恩哭哭采纳,获得10
8秒前
高中生完成签到,获得积分10
9秒前
ChrisKim发布了新的文献求助10
9秒前
博士发布了新的文献求助10
9秒前
10秒前
ttttsy完成签到 ,获得积分10
10秒前
10秒前
10秒前
11秒前
12秒前
朱文琛完成签到,获得积分10
13秒前
tuanzi完成签到 ,获得积分20
13秒前
伶俐绮发布了新的文献求助10
13秒前
Ruby发布了新的文献求助10
13秒前
14秒前
14秒前
SciGPT应助小麻花采纳,获得30
14秒前
14秒前
ZZC发布了新的文献求助30
14秒前
依依完成签到 ,获得积分10
15秒前
xiutang完成签到 ,获得积分10
16秒前
韩涵发布了新的文献求助10
16秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Effective Learning and Mental Wellbeing 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3976058
求助须知:如何正确求助?哪些是违规求助? 3520294
关于积分的说明 11202245
捐赠科研通 3256804
什么是DOI,文献DOI怎么找? 1798471
邀请新用户注册赠送积分活动 877610
科研通“疑难数据库(出版商)”最低求助积分说明 806496