Rapid analysis of flavonoids based on spectral library development in positive ionization mode using LC-HR-ESI-MS/MS

化学 黄酮类 色谱法 串联质谱法 质谱法 类黄酮 高分辨率 异黄酮素 液相色谱-质谱法 有机化学 生物化学 遥感 地质学 抗氧化剂
作者
Nudrat Aziz,Muhammad Khan,Arslan Ali,Adeeba Khadim,Abdullatif Bin Muhsinah,Jalal Uddin,Syed Ghulam Musharraf
出处
期刊:Arabian Journal of Chemistry [Elsevier]
卷期号:15 (4): 103734-103734 被引量:11
标识
DOI:10.1016/j.arabjc.2022.103734
摘要

Natural product screening in plants has always been a difficult task due to the complex nature of the plant material and diverse structures of the compounds present in them. Flavonoids are important and diverse class of plant secondary metabolites with numerous medicinal activities. The present study focuses on the development of a high-resolution tandem mass spectral library for the rapid and authentic identification of common flavonoids. A total of forty flavonoid standards belong to class flavones, isoflavones, flavanones, flavanols and anthocyanins were pooled into two solutions applying logP-based strategy. The flavonoids were analyzed using LC-QTOF-MS high-resolution mass spectrometer with optimization of different instrumental parameters to achieve good sensitivity. The library was built by incorporating names, molecular formulae, exact masses, and MS, and MS/MS spectra of analyzed flavonoids using Bruker Library Editor tool. The fragmentation pattern observed for the standard compounds were compared to the fragments reported in the literature. To assess the practical implications, an extract of tea sample was analyzed and screened using the developed library, which resulted in the identification of three common flavonoids based on their HR-ESI-MS/MS spectral features. The established LC-HR-MS/MS method can be used for the targeted identification of flavonoids in complex samples like food material from different botanical families.
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