淋巴管新生
自噬
细胞生物学
基因沉默
生物
线粒体
ATG5型
淋巴管内皮
脂质代谢
淋巴系统
基因
生物化学
遗传学
细胞凋亡
免疫学
癌症
转移
作者
Odeta Meçe,Diede Houbaert,Maria Livia Sassano,Tania Durré,Hannelore Maes,Marco B.E. Schaaf,Sanket More,Maarten Ganne,Melissa García‐Caballero,Mila Borri,Jelle Verhoeven,Madhur Agrawal,Kathryn Jacobs,Gabriele Bergers,Silvia Blacher,Bart Ghesquière,Mieke Dewerchin,Johannes V. Swinnen,Stefan Vinckier,Marı́a S. Soengas,Peter Carmeliet,Agnès Noël,Patrizia Agostinis
标识
DOI:10.1038/s41467-022-30490-6
摘要
Abstract Autophagy has vasculoprotective roles, but whether and how it regulates lymphatic endothelial cells (LEC) homeostasis and lymphangiogenesis is unknown. Here, we show that genetic deficiency of autophagy in LEC impairs responses to VEGF-C and injury-driven corneal lymphangiogenesis. Autophagy loss in LEC compromises the expression of main effectors of LEC identity, like VEGFR3, affects mitochondrial dynamics and causes an accumulation of lipid droplets (LDs) in vitro and in vivo. When lipophagy is impaired, mitochondrial ATP production, fatty acid oxidation, acetyl-CoA/CoA ratio and expression of lymphangiogenic PROX1 target genes are dwindled. Enforcing mitochondria fusion by silencing dynamin-related-protein 1 (DRP1) in autophagy-deficient LEC fails to restore LDs turnover and lymphatic gene expression, whereas supplementing the fatty acid precursor acetate rescues VEGFR3 levels and signaling, and lymphangiogenesis in LEC-Atg5 −/− mice. Our findings reveal that lipophagy in LEC by supporting FAO, preserves a mitochondrial-PROX1 gene expression circuit that safeguards LEC responsiveness to lymphangiogenic mediators and lymphangiogenesis.
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