亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Structure-immunogenicity relationship of melittin, its transposed analogues, and D-melittin.

蜂毒肽 免疫原性 表位 化学 生物化学 分子生物学 生物 抗原 免疫学
作者
Te Piao King,David Wade,Maria Rosaria Coscia,Scott A. Mitchell,Loucia Kochoumian,Bruce Merrifield
出处
期刊:Journal of Immunology [The American Association of Immunologists]
卷期号:153 (3): 1124-1131 被引量:30
标识
DOI:10.4049/jimmunol.153.3.1124
摘要

Abstract Melittin, a 26-residue bee venom peptide, is known to induce murine Abs specific for its hydrophilic C-terminus of residues 20-26 and T cell responses specific for its hydrophobic mid-region of residue 11-19. Synthetic melittin analogues with transposed sequences of Ac(21-26) (1-20) and Ac(26-21) (1-20) are found to induce murine Abs specific for the transposed peptide segment and to induce T cell responses that are cross-reactive with melittin. Compared with melittin, the transposed melittin analogues are weaker immunogens and have lower hemolytic activities, lower helical contents, and a lower degree of association in micelles. A melittin analogue with a lactoside group at its C-terminus was found to induce lactoside-specific murine Abs. Present studies show that another analogue with a lactoside group at its N-terminus induces only Abs specific for the C-terminal region of melittin, and no lactoside-specific Abs are detected. These immunochemical observations suggest that the immunogenicity of melittin or its analogues is a consequence of its binding to cell membranes with subsequent oligomer formation in lipid bilayers. Apparently melittin or its analogues bind to cell membrane in an asymmetric manner with the exposed and the buried segments functioning as B and T cell epitopes, respectively. D-melittin is non-immunogenic in mice, although D-melittin has the same hemolytic activity as melittin. This finding may be correlated with the known resistance of D-melittin to proteolysis and hence to processing for Ag presentation to T lymphocytes.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
乐乐应助泡面小猪采纳,获得10
18秒前
愤怒的豆腐人完成签到,获得积分10
23秒前
灵溪完成签到 ,获得积分10
23秒前
我有乖乖吃饭完成签到,获得积分20
24秒前
小蘑菇应助我有乖乖吃饭采纳,获得60
33秒前
36秒前
kk发布了新的文献求助10
41秒前
kk完成签到,获得积分10
48秒前
oleskarabach完成签到,获得积分20
59秒前
1分钟前
泡面小猪发布了新的文献求助10
1分钟前
1分钟前
芒果完成签到 ,获得积分10
1分钟前
1分钟前
隐形曼青应助科研通管家采纳,获得10
1分钟前
orixero应助科研通管家采纳,获得10
1分钟前
1分钟前
呜呜老婆完成签到 ,获得积分10
1分钟前
可靠的寒风完成签到,获得积分10
2分钟前
2分钟前
2分钟前
难过的长颈鹿完成签到,获得积分10
2分钟前
2分钟前
2分钟前
2分钟前
2分钟前
wanci应助欢呼的寻双采纳,获得10
2分钟前
3分钟前
ONION发布了新的文献求助10
3分钟前
赘婿应助科研通管家采纳,获得10
3分钟前
4分钟前
4分钟前
5分钟前
jimmy_bytheway完成签到,获得积分0
5分钟前
5分钟前
5分钟前
半。。发布了新的文献求助10
5分钟前
5分钟前
半。。完成签到,获得积分10
5分钟前
丘比特应助科研通管家采纳,获得10
5分钟前
高分求助中
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
Rechtsphilosophie 1000
Bayesian Models of Cognition:Reverse Engineering the Mind 888
Le dégorgement réflexe des Acridiens 800
Defense against predation 800
Very-high-order BVD Schemes Using β-variable THINC Method 568
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3137011
求助须知:如何正确求助?哪些是违规求助? 2787960
关于积分的说明 7784091
捐赠科研通 2444041
什么是DOI,文献DOI怎么找? 1299643
科研通“疑难数据库(出版商)”最低求助积分说明 625497
版权声明 600989