化学
色谱法
十二烷基硫酸钠
聚丙烯酰胺凝胶电泳
聚丙烯酰胺
凝胶电泳
钠
电泳
生物化学
等电点
肺表面活性物质
分子量大小标记
作者
Sanford A. Lacks,Sylvia S. Springhorn,Allan Rosenthal
标识
DOI:10.1016/0003-2697(79)90241-0
摘要
The extent of renaturation of enzymes after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate depended on the source of the detergent. Analysis of commercial preparations of sodium dodecyl sulfate revealed appreciable amounts of tetradecyl and hexadecyl sulfates in some preparations. Inhibition of renaturation was correlated with the amount of hexadecyl sulfate and, to a much lesser extent, of tetradecyl sulfate present. The higher alkyl sulfates appeared to bind more tenaciously to proteins in the gel. More extensive washing was required to remove them than to remove dodecyl sulfate, and they were inhibitory to enzyme activity at lower detergent concentrations. A system is described for gas chromatographic analysis of alkyl sulfates containing chains of 10 to 16 carbon atoms in length.
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