Immobilization of Crosslinked Peptides that Possess High Helical Contents and Their Binding to Target DNAs on Au Surfaces

We designed and synthesized two new types of crosslinked helical peptides that interact with the target DNA. One of them was crosslinked with a conventional isophthalic acid-based agent and was modified with α-lipoic acid at the N-terminus. The other peptide was crosslinked with a novel isophthalic acid-based agent containing α-lipoic acid at its own 5-position. Quartz crystal microbalance analyses showed that both the helical peptides strongly bound to their target DNAs on the Au surface.