Cell‐Free Transcription/Translation from PCR‐Amplified DNA for High‐Throughput NMR Studies

Quick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z605237_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.