Capillary zone electrophoresis with a linear, non-cross-linked polyacrylamide gel: separation of proteins according to molecular mass

Abstract The separation of proteins according to their molecular masses using capillary zone electrophoresis was demonstrated in a buffer containing a linear, non-cross-linked polyacrylamide gel as a sieving medium. The four test proteins, covering a molecular mass range of between 17 800 and 77 000, were applied as sodium dodecyl sulphate (SDS) complexes. The separation was carried out at pH 5.5 (phosphate buffer, 0.5% SDS, 10% liquid, linear polyacrylamide gel). The retention times of the proteins correlated with the logarithm of their molecular masses.