A porous tissue engineering scaffold selectively degraded by cell-generated reactive oxygen species

生物材料 组织工程 活性氧 脚手架 PLGA公司 体内 生物物理学 材料科学 生物医学工程 化学 体外 生物化学 有机化学 医学 生物 生物技术
作者
John R. Martin,Mukesh Kumar Gupta,Jonathan Page,Fang Yu,Jeffrey M. Davidson,Scott A. Guelcher,Craig L. Duvall
出处
期刊:Biomaterials [Elsevier BV]
卷期号:35 (12): 3766-3776 被引量:148
标识
DOI:10.1016/j.biomaterials.2014.01.026
摘要

Biodegradable tissue engineering scaffolds are commonly fabricated from poly(lactide-co-glycolide) (PLGA) or similar polyesters that degrade by hydrolysis. PLGA hydrolysis generates acidic breakdown products that trigger an accelerated, autocatalytic degradation mechanism that can create mismatched rates of biomaterial breakdown and tissue formation. Reactive oxygen species (ROS) are key mediators of cell function in both health and disease, especially at sites of inflammation and tissue healing, and induction of inflammation and ROS are natural components of the in vivo response to biomaterial implantation. Thus, polymeric biomaterials that are selectively degraded by cell-generated ROS may have potential for creating tissue engineering scaffolds with better matched rates of tissue in-growth and cell-mediated scaffold biodegradation. To explore this approach, a series of poly(thioketal) (PTK) urethane (PTK-UR) biomaterial scaffolds were synthesized that degrade specifically by an ROS-dependent mechanism. PTK-UR scaffolds had significantly higher compressive moduli than analogous poly(ester urethane) (PEUR) scaffolds formed from hydrolytically-degradable ester-based diols (p < 0.05). Unlike PEUR scaffolds, the PTK-UR scaffolds were stable under aqueous conditions out to 25 weeks but were selectively degraded by ROS, indicating that their biodegradation would be exclusively cell-mediated. The in vitro oxidative degradation rates of the PTK-URs followed first-order degradation kinetics, were significantly dependent on PTK composition (p < 0.05), and correlated to ROS concentration. In subcutaneous rat wounds, PTK-UR scaffolds supported cellular infiltration and granulation tissue formation, followed first-order degradation kinetics over 7 weeks, and produced significantly greater stenting of subcutaneous wounds compared to PEUR scaffolds. These combined results indicate that ROS-degradable PTK-UR tissue engineering scaffolds have significant advantages over analogous polyester-based biomaterials and provide a robust, cell-degradable substrate for guiding new tissue formation.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
大观天下发布了新的文献求助10
3秒前
忽远忽近的她完成签到 ,获得积分10
3秒前
维生素发布了新的文献求助10
4秒前
butterfly发布了新的文献求助10
6秒前
豆豆完成签到 ,获得积分10
7秒前
范先生完成签到,获得积分10
10秒前
2222222完成签到,获得积分10
10秒前
Hello应助bulingbuling采纳,获得10
10秒前
蜡笔小新完成签到,获得积分10
13秒前
希望天下0贩的0应助小王采纳,获得10
13秒前
赘婿应助lh采纳,获得10
14秒前
14秒前
科研通AI2S应助butterfly采纳,获得10
15秒前
大模型应助butterfly采纳,获得10
15秒前
17秒前
做个梦给你完成签到,获得积分10
17秒前
学霸宇大王完成签到 ,获得积分10
17秒前
甜蜜的楷瑞完成签到,获得积分10
18秒前
魏煜佳完成签到,获得积分10
19秒前
Lc完成签到,获得积分10
19秒前
三伏天完成签到,获得积分10
19秒前
清图完成签到,获得积分10
19秒前
英姑应助简单采纳,获得10
19秒前
爱喝牛奶的大兔子完成签到 ,获得积分20
20秒前
21秒前
21秒前
潇湘雪月完成签到,获得积分10
22秒前
迎南完成签到,获得积分10
22秒前
懒癌晚期完成签到,获得积分10
23秒前
23秒前
初夏微凉发布了新的文献求助10
23秒前
23秒前
24秒前
25秒前
乐乐应助科研通管家采纳,获得10
26秒前
深情安青应助科研通管家采纳,获得10
26秒前
赘婿应助科研通管家采纳,获得10
26秒前
英姑应助科研通管家采纳,获得10
26秒前
田様应助科研通管家采纳,获得10
26秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
Research on Disturbance Rejection Control Algorithm for Aerial Operation Robots 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038388
求助须知:如何正确求助?哪些是违规求助? 3576106
关于积分的说明 11374447
捐赠科研通 3305798
什么是DOI,文献DOI怎么找? 1819322
邀请新用户注册赠送积分活动 892672
科研通“疑难数据库(出版商)”最低求助积分说明 815029