期刊:Advances in Insect Physiology日期:2018-01-01卷期号:: 123-162被引量:8
标识
DOI:10.1016/bs.aiip.2018.07.002
摘要
Abstract The baculovirus expression vector system (BEVS) is currently an important and versatile biotechnological platform for protein expression, particularly for large protein complexes, such as viral-like particles (VLPs). However, baculoviruses seem to show limited potential to be developed as vectors for virus-induced gene silencing, a technique that was originally developed to rapidly analyse gene function in plants. On the other hand, reverse genetics systems of insect RNA viruses can be proposed to function as a biotechnological platform for the production of RNA silencing vectors in insects. RNA viruses that can be considered are nonenveloped viruses with positive-sense ssRNA genome (nodavirus, tetravirus, dicistrovirus, macula-like virus) and with segmented dsRNA genome (cytoplasmic polyhedrosis virus, entomobirnavirus). Engineering of the RNA-dependent RNA polymerase and the viral suppressor of RNAi proteins may accomplish the generation of viral vectors that differ in their persistence/pathogenicity levels for multiple applications in RNA silencing (from specific gene function analysis to insect pest control). VLPs based on the capsid shell and associated proteins of viruses with segmented dsRNA genome also could provide a safer alternative for dsRNA delivery. In such case, the BEVS and MultiBac technology may provide an ideal system for production.