Recent progress in the development of fluorescent probes for detection of biothiols

Biothiols derivatives including cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) play critical roles in maintaining physiological and pathological processes. Developing optical probes for biothiols has attracted great attention owing to their excellent properties like high sensitivity, fast response times and invasive on-time imaging. However, discrimination of Cys, Hcy, and GSH present great challengings due to the similar structures and reactivities of these thiols. This review focuses on the design of fluorescent and colorimetric probes for biothiol detection. Herein, we classify the fluorescent probes for detection biothiols based on various fluorophores like BODIPY, rhodamine, tetraphenylethylene, coumarin, naphthalimide and polymethine. The sensing mechanisms such as Michael addition, cyclization with aldehydes, conjugate addition-cyclization with acrylates, native chemical ligation, cleavage of sulfonamide and sulfonate esters were also included in this review. We hope that much more biothiols fluorescent probes could be explored through the inspiration of this review.