头孢噻肟
头孢菌素
抗生素
微生物学
环丙沙星
大肠杆菌
哌拉西林
拉曼光谱
生物
细菌
生物化学
遗传学
基因
光学
物理
铜绿假单胞菌
作者
Theresa Götz,Marcel Dahms,Johanna Kirchhoff,Claudia Beleites,U. Gläser,Jürgen A. Bohnert,Mathias W. Pletz,Jürgen Popp,Peter Schlattmann,Ute Neugebauer
标识
DOI:10.1002/jbio.202000149
摘要
Abstract A Raman‐based, strain‐independent, semi‐automated method is presented that allows the rapid (<3 hours) determination of antibiotic susceptibility of bacterial pathogens isolated from clinical samples. Applying a priori knowledge about the mode of action of the respective antibiotic, we identified characteristic Raman marker bands in the spectrum and calculated batch‐wise weighted sum scores from standardized Raman intensity differences between spectra of antibiotic exposed and nonexposed samples of the same strains. The lead substances for three relevant antibiotic classes (fluoroquinolone ciprofloxacin, third‐generation cephalosporin cefotaxime, ureidopenicillin piperacillin) against multidrug‐resistant Gram‐negative bacteria (MRGN) revealed a high sensitivity and specificity for the susceptibility testing of two Escherichia coli laboratory strains and 12 clinical isolates. The method benefits from the parallel incubation of control and treated samples, which reduces the variance due to alterations in cultivation conditions and the standardization of differences between batches leading to long‐term comparability of Raman measurements.
科研通智能强力驱动
Strongly Powered by AbleSci AI