A novel antisense long non‐coding RNA participates in asexual and sexual reproduction by regulating the expression of GzmetE in Fusarium graminearum

生物 分生孢子 有性生殖 反义RNA RNA干扰 基因 抄写(语言学) 核糖核酸 突变体 细胞生物学 基因表达 遗传学 微生物学 语言学 哲学
作者
Jie Wang,Wenping Zeng,Jiǎtāo Xiè,Yànpíng Fù,Dàohóng Jiāng,Yang Lin,Weidong Chen,Jiāsēn Chéng
出处
期刊:Environmental Microbiology [Wiley]
卷期号:23 (9): 4939-4955 被引量:7
标识
DOI:10.1111/1462-2920.15399
摘要

Fusarium graminearum is an important worldwide pathogen that causes Fusarium head blight in wheat, barley, maize and other grains. LncRNAs play important roles in many biological processes, but little is known about their functions and mechanisms in filamentous fungi. Here, we report that a natural antisense RNA, GzmetE-AS, is transcribed from the opposite strand of GzmetE. GzmetE encodes a homoserine O-acetyltransferase, which is important for sexual development and plant infection. The expression of GzmetE-AS was increased significantly during the conidiation stage, while GzmetE was upregulated in the late stage of sexual reproduction. Overexpression of GzmetE-AS inhibited the transcription of GzmetE. In contrast, the expression of GzmetE was significantly increased in GzmetE-AS transcription termination strain GzmetE-AS-T. Furthermore, GzmetE-AS-T produced more perithecia and facilitated the ascospore discharge, resembling the phenotype of GzmetE overexpressing strains. However, overexpression of GzmetE-AS in ∆dcl1/2 strain cannot inhibit the expression of GzmetE, and the GzmetE nat-siRNA is also significantly reduced in ∆dcl1/2 mutant. Taken together, we have identified a novel antisense lncRNA GzmetE-AS, which is involved in asexual and sexual reproduction by regulating its antisense gene GzmetE through RNAi pathway. Our findings reveal that the lncRNA plays critical roles in the development of F. graminearum.
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