Deconstruction of Heterogeneity of Size-Dependent Exosome Subpopulations from Human Urine by Profiling N-Glycoproteomics and Phosphoproteomics Simultaneously

化学 磷酸蛋白质组学 外体 微泡 蛋白质组学 糖蛋白 糖蛋白组学 糖基化 蛋白质组 聚糖 色谱法 磷酸化 串联质谱法 质谱法 生物化学 蛋白质磷酸化 小RNA 蛋白激酶A 基因
作者
Haoyang Zheng,Sheng Guan,Xuantang Wang,Jiandong Zhao,Mingxia Gao,Xiangmin Zhang
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:92 (13): 9239-9246 被引量:84
标识
DOI:10.1021/acs.analchem.0c01572
摘要

The heterogeneous populations of exosomes with distinct nanosize have impeded our understanding of their corresponding function as intercellular communication agents. Profiling signaling proteins packaged in each size-dependent subtype can disclose this heterogeneity of exosomes. Herein, new strategy was developed for deconstructing heterogeneity of distinct-size urine exosome subpopulations by profiling N-glycoproteomics and phosphoproteomics simultaneously. Two-dimension size exclusion liquid chromatography (SEC) was utilized to isolate large exosomes (L-Exo), medium exosomes (M-Exo), and small exosomes (S-Exo) from human urine samples. Then, hydrophilic carbonyl-functionalized magnetic zirconium-organic framework (CFMZOF) was developed as probe for capturing the two kinds of post-translational modification (PTM) peptides simultaneously. Finally, liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with database search was used to characterize PTM protein contents. We identified 144 glycoproteins and 44 phosphoproteins from L-Exo, 156 glycoproteins, and 46 phosphoproteins from M-Exo and 134 glycoproteins and 10 phosphoproteins from S-Exo. The ratio of the proteins with simultaneous glycosylation and phosphorylation is 11%, 9%, and 3% in L-Exo, M-Exo, and S-Exo, respectively. Based on label-free quantification intensity results, both principal component analysis and Pearson's correlation coefficients indicate that distinct-size exosome subpopulations exist significant differences in PTM protein contents. Analysis of high abundance PTM proteins in each exosome subset reveals that the preferentially packaged PTM proteins in L-Exo, M-Exo, and S-Exo are associated with immune response, biological metabolism, and molecule transport processes, respectively. Our PTM proteomics study based on size-dependent exosome subtypes opens a new avenue for deconstructing the heterogeneity of exosomes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
1秒前
Lucas应助小甘采纳,获得10
1秒前
和春住完成签到,获得积分10
2秒前
qq完成签到,获得积分10
2秒前
Jarvi发布了新的文献求助10
2秒前
烤鸭完成签到 ,获得积分10
3秒前
zimablue发布了新的文献求助10
3秒前
祝愿完成签到,获得积分10
4秒前
旦皋发布了新的文献求助10
4秒前
4秒前
烟花应助啵萝味儿的奶盖采纳,获得10
5秒前
vincentbioinfo完成签到,获得积分10
5秒前
WJH发布了新的文献求助10
5秒前
量子星尘发布了新的文献求助10
7秒前
12we完成签到 ,获得积分10
8秒前
8秒前
9秒前
学不懂数学应助茶茶采纳,获得20
9秒前
隐形曼青应助旧辞采纳,获得10
9秒前
何佳完成签到,获得积分10
10秒前
烟花应助coco采纳,获得10
10秒前
小晶完成签到,获得积分10
10秒前
zimablue完成签到,获得积分10
11秒前
慕青应助范先生采纳,获得10
11秒前
zzz完成签到,获得积分10
12秒前
13秒前
海盗船长完成签到,获得积分10
13秒前
等待寄云完成签到 ,获得积分10
13秒前
酷波er应助王冉冉采纳,获得10
14秒前
lcjynwe完成签到,获得积分10
15秒前
新奇完成签到 ,获得积分10
15秒前
Misty_发布了新的文献求助10
15秒前
iNk应助不会取名字采纳,获得20
15秒前
Orange应助Hannes采纳,获得10
15秒前
17秒前
多多少少忖测的情完成签到,获得积分10
17秒前
小马甲应助lx采纳,获得10
17秒前
18秒前
阔达冰兰发布了新的文献求助10
18秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
Research on Disturbance Rejection Control Algorithm for Aerial Operation Robots 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038524
求助须知:如何正确求助?哪些是违规求助? 3576221
关于积分的说明 11374737
捐赠科研通 3305912
什么是DOI,文献DOI怎么找? 1819354
邀请新用户注册赠送积分活动 892688
科研通“疑难数据库(出版商)”最低求助积分说明 815048