亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

DIFFERENTIAL DIAGNOSTICS OF GASTRIC CANCER AND PRECANCEROUS CHANGES OF THE GASTRIC MUCOSA USING ANALYSIS OF EXPRESSION OF SIX MICRORNAS

发育不良 癌症 医学 胃粘膜 胃肠病学 内科学 病理 鉴别诊断 腺癌
作者
Serguey V. Titov,V. V. Anishchenko,Tatiana L Poloz,Yulia A Veryaskina,A. A. Arkhipova,S.V. Ustinov
出处
期刊:Klinicheskaia laboratornaia diagnostika [EKOlab]
被引量:5
标识
DOI:10.18821/0869-2084-2020-65-2-131-136
摘要

The lack of specific symptoms for the early detection of gastric cancer leads to the fact that it is often diagnosed at a late stage, when the prognosis is unfavorable. The analysis of molecular markers in addition to standard diagnostic procedures is a promising approach for improving the preoperative diagnosis of both gastric cancer and precancerous changes in the mucosa. Therefore, the aim of our study was to analyze the diagnostic significance of using miRNA expression to diagnosis gastric cancer and precancerous conditions (dysplasia) in histological material. In this work, 122 samples of archival histological material in the form of paraffin blocks were used: 34 samples of gastric adenocarcinoma, 54 samples of gastric ulcers with dysplasia and 34 samples of normal gastric mucosa obtained from patients after bariatric surgery. The expression level of miRNA-145-5p, -150-5p, -20a-5p, -21-5p, -31-5p, -34a-5p, -375 was determined using real-time RT-PCR. Samples were stratified into different groups using the C-RT decision tree algorithm. All miRNAs, except miRNA-20a, were included in the decision tree, which allows stratification of samples for normal mucosa, dysplasia, and gastric cancer. Normal mucosa can be distinguished from gastric cancer only by miRNA-34a, -21, -375. Diagnostic characteristics for the detection of dysplasia: specificity - 97%, sensitivity - 87%; for the detection of gastric cancer: specificity - 91%, sensitivity - 93%. The sufficiently high values of the diagnostic characteristics for detecting dysplasia of the gastric mucosa and gastric cancer obtained in our study indicate the possibility of using expression data of a small amount of miRNAs for the effective separation of samples with tumor and precancerous changes in the stomach tissue.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
量子星尘发布了新的文献求助10
12秒前
机灵自中发布了新的文献求助10
35秒前
机灵自中完成签到,获得积分10
52秒前
执着的草丛完成签到,获得积分10
1分钟前
1分钟前
Anna完成签到 ,获得积分10
1分钟前
1分钟前
绝世冰淇淋完成签到 ,获得积分10
1分钟前
科研通AI5应助科研通管家采纳,获得50
1分钟前
breeze完成签到,获得积分10
1分钟前
量子星尘发布了新的文献求助10
1分钟前
1分钟前
顺心蜜粉给远航的求助进行了留言
1分钟前
silence发布了新的文献求助10
1分钟前
zhanglq发布了新的文献求助10
1分钟前
silence完成签到,获得积分10
2分钟前
CipherSage应助zhaoaotao采纳,获得10
2分钟前
2分钟前
2分钟前
zhaoaotao发布了新的文献求助10
2分钟前
科研通AI5应助祖之微笑采纳,获得10
2分钟前
zhaoaotao完成签到,获得积分10
2分钟前
2分钟前
量子星尘发布了新的文献求助10
2分钟前
2分钟前
隐形曼青应助Master采纳,获得10
2分钟前
3分钟前
科研通AI5应助小嘉采纳,获得10
3分钟前
3分钟前
小嘉发布了新的文献求助10
3分钟前
大个应助哈尔滨采纳,获得10
3分钟前
英姑应助科研通管家采纳,获得10
3分钟前
郗妫完成签到,获得积分10
3分钟前
顺心蜜粉完成签到,获得积分10
3分钟前
3分钟前
Master发布了新的文献求助10
3分钟前
英俊的铭应助Magali采纳,获得10
3分钟前
4分钟前
4分钟前
祖之微笑发布了新的文献求助10
4分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3976649
求助须知:如何正确求助?哪些是违规求助? 3520756
关于积分的说明 11204743
捐赠科研通 3257502
什么是DOI,文献DOI怎么找? 1798733
邀请新用户注册赠送积分活动 877897
科研通“疑难数据库(出版商)”最低求助积分说明 806629