Nontarget Screening of Per- and Polyfluoroalkyl Substances Binding to Human Liver Fatty Acid Binding Protein

化学 结合亲和力 亲缘关系 脂肪酸结合蛋白 洗脱 电喷雾电离 色谱法 立体化学 生物化学 质谱法 受体 基因
作者
Diwen Yang,Jiajun Han,David Ross Hall,Jianxian Sun,Jesse Fu,Steven Kutarna,Keith A. Houck,Carlie A. LaLone,Jon A. Doering,Carla A. Ng,Hui Peng
出处
期刊:Environmental Science & Technology [American Chemical Society]
卷期号:54 (9): 5676-5686 被引量:62
标识
DOI:10.1021/acs.est.0c00049
摘要

More than 1000 per- and polyfluoroalkyl substances (PFASs) have been discovered by nontarget analysis (NTA), but their prioritization for health concerns is challenging. We developed a method by incorporating size-exclusion column co-elution (SECC) and NTA, to screen PFASs binding to human liver fatty acid binding protein (hL-FABP). Of 74 PFASs assessed, 20 were identified as hL-FABP ligands in which eight of them have high binding affinities. Increased PFAS binding affinities correlate with stronger responses in electrospray ionization (ESI-) and longer retention times on a C18 column. This is well explained by a mechanistic model, which revealed that both polar and hydrophobic interactions are crucial for binding affinities. Encouraged by this, we then developed an SECC method to identify hL-FABP ligands, and all eight high-affinity ligands were selectively captured from 74 PFASs. The method was further applied to an aqueous film-forming foam (AFFF) product in which 31 new hL-FABP ligands were identified. Suspect and nontargeted screening revealed these ligands as analogues of perfluorosulfonic acids and homologues of alkyl ether sulfates (C8- and C10/EOn, C8H17(C2H4O)nSO4-, and C10H21(C2H4O)nSO4-). The SECC method was then applied to AFFF-contaminated surface waters. In addition to perfluorooctanesulfonic acid and perfluorohexanesulfonic acid, eight other AFFF chemicals were discovered as novel ligands, including four C14- and C15/EOn. This study implemented a high-throughput method to prioritize PFASs and revealed the existence of many previously unknown hL-FABP ligands.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
发nature完成签到 ,获得积分10
刚刚
1秒前
1秒前
今后应助欣慰的乌冬面采纳,获得10
3秒前
FashionBoy应助xsuvian采纳,获得10
3秒前
CodeCraft应助wtt采纳,获得10
3秒前
Owen应助wtt采纳,获得10
4秒前
bkagyin应助wtt采纳,获得10
4秒前
大模型应助wtt采纳,获得10
4秒前
脑洞疼应助wtt采纳,获得10
4秒前
Lucas应助wtt采纳,获得10
4秒前
脑洞疼应助wtt采纳,获得10
4秒前
大模型应助wtt采纳,获得10
4秒前
上官若男应助wtt采纳,获得10
4秒前
我是老大应助wtt采纳,获得10
4秒前
6秒前
7秒前
桐桐应助notsoeasy采纳,获得10
9秒前
9秒前
10秒前
10秒前
Jungel完成签到,获得积分0
10秒前
10秒前
11秒前
滑步小镰刀关注了科研通微信公众号
12秒前
灵魂发布了新的文献求助10
13秒前
柯米克发布了新的文献求助10
14秒前
15秒前
15秒前
www完成签到,获得积分10
15秒前
xsuvian发布了新的文献求助10
16秒前
澡雪发布了新的文献求助10
16秒前
紧张的妖妖完成签到,获得积分20
17秒前
南宫书瑶发布了新的文献求助10
18秒前
18秒前
Lucas应助jignjing采纳,获得10
19秒前
普贤行完成签到,获得积分10
19秒前
20秒前
艾小晗完成签到,获得积分10
25秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975661
求助须知:如何正确求助?哪些是违规求助? 3520000
关于积分的说明 11200535
捐赠科研通 3256410
什么是DOI,文献DOI怎么找? 1798247
邀请新用户注册赠送积分活动 877490
科研通“疑难数据库(出版商)”最低求助积分说明 806390