电泳
Zeta电位
表面电荷
分析化学(期刊)
凝胶电泳
多普勒效应
化学
材料科学
色谱法
纳米技术
物理
纳米颗粒
天文
生物化学
物理化学
作者
Fairuzeta Ja’afar,Chee Hau Leow,Valeria Garbin,Charles A. Sennoga,Meng‐Xing Tang,John M. Seddon
标识
DOI:10.1016/j.ultrasmedbio.2015.07.001
摘要
Microbubble (MB) contrast-enhanced ultrasonography is a promising tool for targeted molecular imaging. It is important to determine the MB surface charge accurately as it affects the MB interactions with cell membranes. In this article, we report the surface charge measurement of SonoVue, Definity and Optison. We compare the performance of the widely used laser Doppler electrophoresis with an in-house micro-electrophoresis system. By optically tracking MB electrophoretic velocity in a microchannel, we determined the zeta potentials of MB samples. Using micro-electrophoresis, we obtained zeta potential values for SonoVue, Definity and Optison of -28.3, -4.2 and -9.5 mV, with relative standard deviations of 5%, 48% and 8%, respectively. In comparison, laser Doppler electrophoresis gave -8.7, +0.7 and +15.8 mV with relative standard deviations of 330%, 29,000% and 130%, respectively. We found that the reliability of laser Doppler electrophoresis is compromised by MB buoyancy. Micro-electrophoresis determined zeta potential values with a 10-fold improvement in relative standard deviation.
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