神经保护
多巴胺能
程序性细胞死亡
神经元
活力测定
细胞凋亡
酪氨酸羟化酶
生物
苯乙醇
化学
多巴胺
细胞生物学
药理学
生物化学
分子生物学
神经科学
糖苷
植物
作者
Yanyun Li,Jianghai Lu,Quan Li,Yuying Zhao,Xiaoping Pu
标识
DOI:10.1016/j.ejphar.2007.10.052
摘要
Parkinson's disease is characterized by the progressive degeneration of midbrain dopaminergic neurons. Buddleia lindleyana is a traditional Chinese herb, commonly called Zui Yu Cao. The purification and identification of pedicularioside A and other phenylethanoid glycosides from this plant have been reported. However, their neuroprotective effects on the 1-methyl-4-phenylpyridinium ion (MPP+)-induced death of rat mesencephalic neuron primary cultures and the precise mechanism of this protection remains unclear. We used the 3-(4, 5-dimethylthiozol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay for cellular growth to examine the effects of five phenylethanoid glycosides isolated from B. lindleyana, including pedicularioside A, leucosceptoside A, isoacteoside, acteoside, and arenariside, on the viability of mesencephalic neurons treated with MPP+. Of the compounds tested, pedicularioside A exhibited the greatest degree of protection from MPP+-induced cell death. We also observed a marked increase in the number of tyrosine hydroxylase immunoreactive neurons. Pedicularioside A inhibited expression of the caspase-3 gene and cleavage of poly (ADP-ribose) polymerase (PARP) in cultures exposed to MPP+. Our results suggest that pedicularioside A has a neuroprotective effect to improve the survival of mesencephalic neurons (dopaminergic neurons and non-dopaminergic neurons). The mode of action appears to be the inhibition of caspase-3 gene expression, thereby protecting mesencephalic neurons from MPP+-induced cell death.
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