展青霉素
遗传毒性
细胞毒性
氧化应激
DNA损伤
背景(考古学)
抗氧化剂
化学
维生素E
彗星试验
药理学
生物
毒性
生物化学
分子生物学
DNA
真菌毒素
食品科学
体外
有机化学
古生物学
作者
Imen Ayed‐Boussema,Haila Abassi,Chayma Bouaziz,Wiem Ben Hlima,Yosra Ayed,Hassen Bacha
摘要
Abstract Patulin (PAT) is a mycotoxin produced in fruits, mainly in apples, by certain species of Penicillium , Aspergillus , and Byssochlamys . It has been shown that PAT is cytotoxic, genotoxic, and mutagenic in different cell types. Several studies incriminate the oxidative stress as a mechanism of PAT‐mediated toxicity. In this context, our aim was to investigate the protective role of Vitamin E (Vit E), an antioxidant agent, against PAT induced cytotoxicity and genotoxicity in cultured HepG2 cells. The obtained results showed that addition of Vit E in cells treated with PAT significantly reduce cell mortality induced by this toxin. In the same conditions, Vit E decreased the intracellular level of ROS, reduced PAT induced p53 expression, and reversed PAT induced DNA damage. In addition, Vit E prevented significantly the percentage of chromosome aberrations induced by PAT in HepG2 cells in a concentration dependant manner. These results suggest that Vit E, an exogenous antioxidant agent, plays an important role in defense against PAT‐induced cytotoxicity and genotoxicity, which confirms the involvement of oxidative stress in the induction of DNA damage by PAT in HepG2 cells. © 2011 Wiley Periodicals, Inc. Environ Toxicol, 2013.
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