成熟
分解代谢抑制
分解代谢
脱镁叶绿酸A
乙烯
更年期
生物化学
叶绿素
基因表达
基因
互补DNA
酶
新陈代谢
生物
化学
植物
遗传学
突变体
更年期
催化作用
作者
Rosybel Drury,Stefan Hörtensteiner,Iain Donnison,Colin R. Bird,Graham B. Seymour
标识
DOI:10.1034/j.1399-3054.1999.100105.x
摘要
The biochemical and molecular basis of chlorophyll (Chl) catabolism in bananas was investigated during ripening at 20°C and at an elevated temperature (35°C) where degreening is inhibited. Biochemical analysis showed that Chl breakdown products could be isolated from fruit ripened at both temperatures. The coloured breakdown products, chlorophyllide and pheophorbide, were not detected at any stage of ripening in the two treatments; however, a non‐fluorescent Chl catabolite accumulated to a higher concentration at 20 than at 35°C. To investigate the ripening‐related gene expression associated with these changes, a cDNA library was generated from the peel of fruit ripened at 20°C. Differential screening of this library produced 20 non‐redundant families of clones including those encoding enzymes involved in ethylene biosynthesis, respiration, starch metabolism, cell wall degradation and other metabolic events. The expression of these genes was followed by northern analysis in fruit ripened at 20 and 35°C.
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