Identification of a New Class of Lipid Droplet-Associated Proteins in Plants

油红素 脂滴 拟南芥 拟南芥 细胞生物学 植物脂质转运蛋白 生物 脂质代谢 生物化学 化学 植物 生物物理学 突变体 基因
作者
Patrick J. Horn,Christopher N. James,Satinder K. Gidda,Aruna Kilaru,John M. Dyer,Robert T. Mullen,John B. Ohlrogge,Kent D. Chapman
出处
期刊:Plant Physiology [Oxford University Press]
卷期号:162 (4): 1926-1936 被引量:160
标识
DOI:10.1104/pp.113.222455
摘要

Abstract Lipid droplets in plants (also known as oil bodies, lipid bodies, or oleosomes) are well characterized in seeds, and oleosins, the major proteins associated with their surface, were shown to be important for stabilizing lipid droplets during seed desiccation and rehydration. However, lipid droplets occur in essentially all plant cell types, many of which may not require oleosin-mediated stabilization. The proteins associated with the surface of nonseed lipid droplets, which are likely to influence the formation, stability, and turnover of this compartment, remain to be elucidated. Here, we have combined lipidomic, proteomic, and transcriptomic studies of avocado (Persea americana) mesocarp to identify two new lipid droplet-associated proteins, which we named LDAP1 and LDAP2. These proteins are highly similar to each other and also to the small rubber particle proteins that accumulate in rubber-producing plants. An Arabidopsis (Arabidopsis thaliana) homolog to LDAP1 and LDAP2, At3g05500, was localized to the surface of lipid droplets after transient expression in tobacco (Nicotiana tabacum) cells that were induced to accumulate triacylglycerols. We propose that small rubber particle protein-like proteins are involved in the general process of binding and perhaps the stabilization of lipid-rich particles in the cytosol of plant cells and that the avocado and Arabidopsis protein members reveal a new aspect of the cellular machinery that is involved in the packaging of triacylglycerols in plant tissues.
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