Wild bird-origin H3N8 avian influenza virus exhibit well adaptation in mammalian host

•A novel reassortant avian influenza A(H3N8) virus was isolated from wild birds. •Several putative mammalian adaptive mutation was occurred in this H3N8 virus. •This H3N8 virus can bind to both avian-type and human-type receptor. •This H3N8 virus obtain the replication ability and exhibit pathogenicity to mammals.